Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
1997-06-12
2001-02-20
Patterson, Jr., Charles L. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S209000
Reexamination Certificate
active
06190890
ABSTRACT:
TECHNICAL FIELD OF THE INVENTION
The present invention relates to novel enzymes. More specifically, the invention relates to novel fungal cellulases and methods of obtaining the DNA encoding them.
BACKGROUND
There is an increasing interest in glucanases in the food and feed industries. These enzymes find application for instance in the fruit juice industry for liquefaction of plant cell wall material (pending application EP 94202442.3). They may also serve as processing aids to reduce fouling of membranes. In the feed industry the role of &bgr;-glucanases in reducing the viscosity of various sorts of grains is well established. Many of the enzyme preparations used in the food and feed area are derived from Aspergillus species, usually
Aspergillus niger.
This is a safe host which produces a large variety of enzymes such as pectinases and hemicellulases with characteristics which make them suitable for applications at moderate temperatures and at neutral to acidic pH. In contrast to pectinases and hemicellulases, cellulases are usually derived from Trichoderma. Trichoderma species such as
reesei, viride
or
longibrachiatum
are good producers of cellulolytic enzymes. However, Trichoderma enzymes cannot be used everywhere due to regulatory constraints. Thus, it would be of considerable economic value to have a good source of Aspergillus enzymes. However, up till now, it has not been possible to clone the genes encoding glucanases from
A. niger
using the traditional method involving enzyme purification, partial amino acid sequencing and isolation of the gene or cDNA for the enzyme of interest by the derived DNA sequence.
SUMMARY
The present invention provides polypeptides with cellulase activity, corresponding DNA sequences, vectors, transformed hosts and a method for the production of these polypeptides which are obtainable from
Aspergillus niger
var. niger or
Aspergillus niger
var. tubigensis.
REFERENCES:
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patent: 0420358 (1990-09-01), None
patent: 96/29415 (1996-09-01), None
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Gokhale, D. V., et. al. (1988) Enzyme Microb. Technol. 10, 442-445.
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Tilburn et al., “Transformation by integration inAspergillus nidulans”, Gene 26 (1983) 205-221.
Kelly et al., “Transformation ofAspergillus nigerby the amdS gene ofAspergillus nidulans”, (1985), EMBO. J. 4,475-479.
Somogyi “Notes on Sugar Determination”, Journal of Biological Chemistry, 1952; 195; 19-23.
Sakamoto et al., “Cloning and sequencing of cellulase DNA fromAspergillus Kawachiiand its expression inSaccharomyces Cerevisiae”, Curr. Genet (1995) 27:435-439.
de Graaff Leendert H.
van den Broeck Henriette C.
Van Ooijen Albert J. J.
Visser Jacob
Gist-Brocades B.V.
Morrison & Foerster / LLP
Patterson Jr. Charles L.
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