Methods and vectors for site-specific recombination

Details Methods and vectors for site-specific recombination Methods and vectors for site-specific recombination

1998-02-25

2000-05-16

Brusca, John S.

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

435455, 4353201, 536 231, 536 2372, 536 241, C12N 1590, C12N 1579, C12N 1534

Patent

active

060636270

ABSTRACT:
The present invention provides methods for site-specific recombination in a cell, as well as vectors which can be employed in such methods. The methods and vectors of the present invention can be used to obtain persistent gene expression in a cell and to modulate gene expression.
One preferred method according to the invention comprises contacting a cell with a vector comprising an origin of replication functional in mammalian cells located between first and second recombining sites located in parallel. Another preferred method comprises, in part, contacting a cell with a vector comprising first and second recombining sites in antiparallel orientations such that the vector is internalized by the cell. In both methods, the cell is further provided with a site-specific recombinase that effects recombination between the first and second recombining sites of the vector.

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