Modified enzymes and methods for making same

Details Modified enzymes and methods for making same Modified enzymes and methods for making same

1984-05-29

1988-07-26

Tarcza, John Edward

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4351723, 435221, 435 91, 4351721, 935 14, 935 10, 252547, C12N 956, C12N 1500, C12N 954, C12P 1934

Patent

active

047600250

ABSTRACT:
A cloned subtilisin gene has been modified at specific sites to cause amino acid substitutions at certain spots in the enzyme. The modified enzyme, preferably produced by Bacillus, is useful in combination with detergents.

REFERENCES:
patent: 3985686 (1976-10-01), Barrat
Paterson et al., "Molecular Basis of Altered Enzyme Specificities in a Family of Mutant Amidases", Journal of General Microbiology 114, pp. 75-85 (1979).
Nedkov et al., "Primary Structure of Subtilisin DY" Hoppe-Seyler's Z. Physiol. Chem. 346(11), pp. 1537-1540 (1983) C.A. 100:81934r.
Kerjan et al., "Characterization of a Thermosensitive Sporulation Mutant of Bacillus subtilis Affected in the Structural Gene" European Journal of Biochemistry 98, pp. 353-362 (1979).
Uehara et al., "Thermosensitive, Extracellular Neutral Proteases in Bacillus subtilis: Isolation, Characterization and Genetics Journal of Bacteriology 139(2), pp. 583-590 (1979).
Polgar et al., "Peptic Peptide of Thiolsubtilisin" Biochimica et Biophysica Acta 667, pp. 351-354 (1981).

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