Catalytic RNA molecules

Details Catalytic RNA molecules Catalytic RNA molecules

1996-07-17

2000-05-16

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 9131, 536 231, 536 232, 536 245, C07H 2104, C12Q 168

Patent

active

060635665

ABSTRACT:
The present invention discloses nucleic acid enzymes capable of cleaving nucleic acid molecules, including single-stranded DNA, in a site-specific manner under physiologic conditions, as well as compositions including same. The present invention also discloses methods of making and using the disclosed enzymes and compositions.
The present invention further discloses nucleic acid enzymes or catalytic (enzymatic) RNA molecules that are capable of cleaving a variety of bonds, including phosphodiester bonds and amide bonds, in a variety of substrates. Thus, various disclosed enzymatic RNA molecules are capable of functioning as nucleases, amidases, and/or peptidases. The present invention also relates to compositions containing the disclosed catalytic RNA molecules and to methods of making, selecting, and using such enzymes and compositions.

REFERENCES:
Branch TIBS 23:45-50, Feb. 1998.
Bartel and Szostak, "Isolation of New Ribozymes from a Large Pool of Random Sequences" Science, 261: 1411-1418 (1993).
Beaudry and Joyce, "Mimimum Secondary Structure Requirements for Catalytic Activity of a Self-Splicing Group I Intron" Biochemistry, 29:6534-6539 (1990).
Beaudry and Joyce, Directed Evolution of an RNA Enzyme, Science, 257: 635-641 (1992).
Breaker and Joyce, "Inventing and Improving Ribozyme Function: Rational Design Versus Iterative Selection Methods" Trends Biotech., 12: 268-275 (1994).
Burke, John M., "Molecular Genetics of Group I Introns: RNA Structures and Protein Factors Required for Splicing--a review" Gene, 73:273-294 (1988).
Cadwell and Joyce, "Randomization of Genes by PCR Mutagenesis" PCR Methods Appl. 2:28-33 (1992).
Cadwell and Joyce, "Mutagenic PCR" PCR Methods and Applications 3 (Suppl): S136-S140 (1994).
Cech, Thomas R., "The Chemistry of Self-Splicing RNA and RNA Enzymes" Science, 236: 1532-1539 (1987).
Chowrira, et al., "Novel Guanosine Requirement for Catalysis by the Hairpin Ribozyme" Nature, 354: 320-322 (1991).
Cwirla, et al., "Peptides on Phage: A Vast Library of Peptides for Identifying Ligands" Proc. Natl. Acad. Sci., USA, 87: 6378-6382 (1990).
Dai, et al., "Cleavage of an Amide Bond by a Ribozyme" Science, 267:237-240 (1995).
Dai, et al., "Cleavage of an Amide Bond by a Ribozyme" J. Cell Biochem. Suppl. 19A:229 (1995).
Devlin, et al., "Random Peptide Libraries: A Source of Specific Protein Binding Molecules" Science, 249: 404-4406 (1990).
Ellington and Szostak, "In Vitro Selection of RNA Molecules that Bind Specific Ligands" Nature, 346: 818-822 (1990).
Green, et al., "In Vitro Genetic Analysis of the Tetrahymena Self-Splicing Intron" Nature, 347:406-408 (1990).
Herschlag and Cech, "DNA Cleavage Catalysed by the Ribozyme from Tetrahymena" Nature, 344: 405-409 (1990).
Herschlag and Cech, "Catalysis of RNA Cleavage by the Tetrahymena Thermophila Ribozyme . . . " Biochemistry, 29:10159-10171 (1990).
Herschlag, et al., "The Importance of Being Ribose at the Cleavage Site in the Tetrahymena Ribozyme Reaction" Biochemistry, 32: 8312-8321 (1993).
Inoue, et al., "New Reactions of the Ribosomal RNA Precursor of Tetrahymena and the Mechanism of Self-Splicing" J. Mol. Biol., 189: 143-165 (1986).
Jellinek, et al., "High-Affinity RNA Ligands to Basic Fibroblast Growth Factor Inhibit Receptor Binding" PNAS, 90: 11227-11231 (1993).
Joyce and Inoue, "Structure of the Catalytic Core of the Tetrahymena Ribozyme as Indicated by Reactive . . . " Nucleic Acids Res., 15: 9825-9840 (1987).
Joyce, Gerald F., "Amplification, Mutation and Selection of Catalytic RNA" Gene, 82: 83-87 (1989).
Joyce and Inoue, "A Novel Technique for the Rapid Preparation of Mutant RNAs" Nucleic Acids Res., 17: 711-722 (1989).
Joyce, et al., "Catalytic Activity is Retained in the Tetrahymena Group I Intron Despite Removal . . . " Nucleic Acids Res., 17: 7879-7889 (1989).
Joyce, Gerald F., "Building the RNA World: Evolution of Catalytic RNA in the Laboratory" Molec. Biol. of RNA: UCLA Symposia on Molecular and Cellular Biology, T.R. Cech (ed.), Liss, NY, 1989, pp. 361-371.
Joyce, Gerald F., "Selective Amplification Techniques for Optimization of Ribozyme Function" Antisense RNA and DNA, J.A.H. Murray (ed.), Wiley-Liss, NY 1992, pp. 353-372.
Joyce, Gerald F., "Evolution of Catalytic Function" Pure & Appl. Chem., 65: 1205-1212 (1993).
Joyce, et al., "Directed Evolution of a Succession of Ribozymes: From RNAse to DNAse to Amidase" FASEB Journ., 9: A1390 (1995).
Lam, et al., "A New Type of Synthetic Peptide Library for Identifying Ligand-Binding Activity" Nature, 354: 82-84 (1991).
Lehman and Joyce, "Evolution in vitro of an RNA Enzyme with Altered Metal Dependence" Nature, 361: 182-185 (1993).
Lorsch, and Szostak, "Chance and Necessity in the Selection of Nucleic Acid Catalysts" Acc. Chem. Res., 29: 103-110 (1996).
Mills, et al., "An Extracellular Darwinian Experiment with a Self-Duplicating Nucleic Acid Molecule" PNAS, 58: 217-224 (1967).
Piccirilli, et al., "Aminoacyl Esterase Activity of the Tetrahymena Ribozyme" Science, 256: 1420-1424 (1992).
Roberts and Crothers, "Stability and Properties of Double and Triple Helices: Dramatic Effects of RNA . . . " Science, 258: 1463-1466 (1992).
Robertson and Joyce, "Selection in vitro of an RNA Enzyme that Specifically Cleaves Single-Stranded DNA" Nature, 344: 467-468 (1990).
Scott and Smith, "Searching for Peptide Ligands with an Epitope Library" Science, 249: 386-390 (1990).
Sugimoto, et al., "Effects of Substrate Structure on the Kinetics of Circle Opening Reactions of the . . . " Nucleic Acids Res., 17: 355-371 (1989).
Tsang and Joyce, "Evolutionary Optimization of the Catalytic Properties of a DNA-Cleaving Ribozyme" Biochemistry, 33: 5966-5973 (1994).
Tuerk, and Gold, "Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase" Science, 249: 505-510 (1990).
Wang and Cech, "Tertiary Structure Around the Guanosine-Binding Site of the Tetrahymena Ribozyme" Science, 256: 526-529 (1992).
Young, et al., "Mutations in a Nonconserved Sequence of the Tetrahymena Ribozyme Increase Activity and Specificity" Cell, 67: 1007-1019 (1991).
Zaug and Cech, "The Intervening Sequence RNA of Tetrahymena is an Enzyme" Science, 231: 470-475 (1986).
Zaug, et al., "The Tetrahymena Ribozime Acts Like an RNA Restriction Endonuclease" Nature, 324: 429-433 (1986).

Affiliated with

Also associated with

Rating

Catalytic RNA molecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Catalytic RNA molecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Catalytic RNA molecules will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-257351