Textiles: fiber preparation
Patent
1977-04-04
1979-02-20
Wiseman, Thomas G.
Textiles: fiber preparation
1951035R, 195127, G01N 3114
Patent
active
041405790
ABSTRACT:
A uniform dispersion of a phospholipid in an aqueous based, salt containing solution may be obtained by dissolving the phospholipid in an alcohol chosen from the group consisting of methanol, ethanol, propanol, and isopropyl alcohol, and adding the phospholipid-alcohol solution to the aqueous based solution. The uniform dispersion will remain, even if the alcohol is evaporated or otherwise removed from the aqueous solution. If lecithin or certain other phospholipids useful in testing for the presence or activity of phospholipase C or A enzymes are uniformly dispersed in this manner, the resulting aqueous dispersion may be solidified by the addition of a hardening agent, such as agar, and the so-solidified dispersion used in an improved testing procedure for such enzymes. The enzymes will cause areas of hydrolysis to occur when placed in wells cut in the solidified dispersion, the size of these areas indicating the amount of enzyme present.
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Ikezawa et al., "Studies on Phosphatidylinositol Phosphodiesterase (Phospholipase C Type) of Bacillus cereus", Biochim. Biophys., Acta, vol. 450 (1976), pp. 154-164.
Hayakawa et al., "Preparation of Artificial Vesicles Having an L-Alanine Uptake Activity Which Requires NAPH as Energy Source", Biochem. and Biophys. Research Com., vol. 72, No. 4 (1976), pp. 1548-1553.
Cooper et al., "Factors Influencing the Lipid Composition and Fluidity of Red Cell Membranes in Vitro: Production of Red Cells Possessing More Than Two Cholesterols per Phospholipid", Biochemistry, vol. 17 (1978), pp. 327-331.
Nagler, "Observations on a Reaction Between the Lethal Toxin of Cl. welchii
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