Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
1999-09-02
2001-07-10
Fredman, Jeffrey (Department: 1655)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S912000, C536S024300
Reexamination Certificate
active
06258542
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of The Invention
The present invention relates to a method for supporting DNA-fixation and a DNA-fixed support, and more particularly to a suitable method for supporting DNA-fixation and a DNA-fixed support used in case of utilizing DNAs in DNA technology industry, life science industry as well as medical and pharmaceutical industry etc.
Furthermore, a method for supporting fixation of RNA or PNA, or other high-molecular fragments with base sequences and a fixed support of RNA or PNA, or other high-molecular fragments with base sequences as well as to a method for delivering and storing DNA or RNA or PNA, or other high-molecular fragments with base sequences.
2. Description of The Related Art
Heretofore, either of a method wherein DNA in a state of aqueous solution is frozen as it stands, or a method wherein DNA is subjected to glycerol stock at −80° C. together with host cells in a state where the DNA is cloned into vector has been made in order to preserve stably DNA for a long period of time.
In the case where DNA which has been preserved by a conventional method as described above is intended to distribute by mailing or the like manner, for example, in the DNA which has been preserved by the former method (the DNA in a state of aqueous solution is frozen as it stands), the following steps must be taken. First, the solution of DNA which has been frozen is unfrozen to prepare a DNA aqueous solution. Thereafter, the resulting prepared DNA aqueous solution is pipetted into a micro-tube (a micro-tube is made of, for example, polypropylene and the like). Furthermore, the DNA aqueous solution which has been pipetted into the micro-tube is dried completely, and then the resulting dried DNA is mailed together with the micro-tube at ordinary temperature.
On one hand, in the DNA which has been preserved by the latter method (DNA is subjected to glycerol stock at −80° C. together with host cells in a state where the DNA is cloned into vector has been made), the following steps must be taken. First, the DNA is extracted from the host cells, and an aqueous solution of the DNA is prepared. After having been prepared the DNA aqueous solution, the resulting prepared DNA aqueous solution is pipetted into a micro-tube, and then the DNA aqueous solution which has been pipetted into the micro-tube is dried completely, thereafter the resulting dried DNA is mailed together with the micro-tube at ordinary temperature as in the case of the former method described above.
Namely, a variety of processes of operation which require comparatively long working hours such as preparation of DNA aqueous solution, pipetting of the DNA aqueous solution into a micro-tube, and drying of the DNA aqueous solution which has been pipetted into the micro-tube must have been carried out in the case where DNA preserved by the above described conventional methods is intended to distribute widely by mailing or the like manner.
In these circumstances, although small number of micro-tubes can be distributed by small number of work force, an amount of working becomes enormous in the case where the number of micro-tubes to be distributed increases, so that there is such a problem that much labor and much time are required, resulting in difficulty in distribution of such micro-tubes as a matter of fact.
Recently, on the other hand, further plenitude of DNA bank system is requested with the development of genome analysis project of a variety of organisms, so that a need for reductions of the labor and time as described above as well as a need for engineering developments for the sake of increased efficiency in operation which are required for preservation and distribution of DNA become remarkable.
Moreover, there are also such problems and needs as described above as to RNA or PNA, or other high-molecular fragments with base sequences.
OBJECTS AND SUMMARY OF THE INVENTION
The present invention has been made in view of the above described problems involved in the prior art and a need for solving such problems in late years.
Accordingly, an object of the present invention is to provide a method for supporting DNA-fixation and DNA-fixed support by which DNA can be promptly and efficiently preserved and distributed without taking much labor and much time.
Another object of the present invention is to provide a method for supporting fixation of RNA or PNA or other high-molecular fragments with base sequences and a fixed support of RNA or PNA or other high-molecular fragments with base sequences by which RNA or PNA or other high-molecular fragments with base sequences can be promptly and efficiently preserved and distributed without taking much labor and much time.
A further object of the present invention is to provide a method for delivering and storing DNA or RNA or PNA or other high-molecular fragments with base sequences.
In order to attain the above described object, a method for supporting DNA-fixation according to the present invention is constituted in such that a DNA solution is allowed to adhere to a sheet-like support having a prescribed thickness, or the DNA solution is allowed to adhere to the sheet-like support by printing, and the DNA solution which has been thus allowed to adhere to the support is dried, whereby the DNA is fixed to the support.
Namely, according to the method for supporting DNA-fixation of the present invention, DNA can be fixed or printed on a sheet-like support by such a simple operation that a DNA solution is allowed to adhere to a sheet-like support, and then the resulting sheet is dried, so that it becomes possible to preserve and distribute promptly and efficiently the DNA without taking much labor and much time.
Furthermore, a DNA-fixed support according to the present invention comprises a sheet-like support having a prescribed thickness, and a DNA fixed or printed to the support by drying a DNA solution which has been allowed to adhere to the support, or printing the DNA solution onto the support.
Thus, according to the DNA-fixed support of the present invention, since a DNA has been fixed or printed onto a sheet-like support, the resulting DNA-fixed support which is a support on which the DNA has been fixed or printed may be mailed without requiring any further treatment. As a result, it becomes possible to distribute promptly and efficiently without taking much labor and much time.
Under the circumstances, the above described support may be prepared from a material containing a site in which a DNA has been fixed or printed, the site being severable.
According to the preparation as described above, since a site in which a DNA has been fixed or printed can be severed, workability in recovering operation of the DNA which has been fixed or printed to the support can be improved.
Furthermore, the above described support may be prepared from cellulose as the major component.
According to the preparation as described above, a DNA fixed or printed to the support can be reliably preserved at ordinary temperature.
Moreover, the above described support may contain a site on which information as to a DNA which has been fixed or printed to the support is described.
According to the preparation as described above, contents of the DNA which has been fixed or printed to a support for DNA-fixation can be confirmed by visual observation of the DNA-fixed support.
Still further, a plurality of the above described supports are laminated to form a booklet, and a DNA may be fixed or printed to each support from which the above described booklet is formed.
According to the preparation as described above, a plurality of DNAs can be promptly and efficiently distributed by mailing the resulting booklet without taking much labor and much time.
Yet further, a DNA which has been fixed or printed to the above described support is arranged in such that the DNA is recovered by elution from the support.
Accordingly, the DNA which has been fixed or printed to the support can be easily recovered.
Still further, the DNA which has been recovered by elution from the above desc
Birch Stewart Kolasch & Birch, LLP.
Chakrabarti Arun
Fredman Jeffrey
The Institute of Physical and Chemical Resaerch (Riken)
LandOfFree
Method for supporting DNA-fixation and DNA-fixed support does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for supporting DNA-fixation and DNA-fixed support, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for supporting DNA-fixation and DNA-fixed support will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2520306