Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
1999-02-24
2001-05-08
Geist, Gary (Department: 1623)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S004100, C536S128000
Reexamination Certificate
active
06228996
ABSTRACT:
BACKGROUND OF THE INVENTION
The invention relates to a process for extracting diterpene glycosides from plant or botanical sources.
Diterpene glycosides are useful as naturally sweet material and can be obtained from various sources.
Typical plant or botanical sources contain useful diterpene glyocosides as well as certain additional polyphenolic substances, or tannins, some of which give health benefits such as anti-inflammation, anti-oxidation and the like. Other portions of these tannins, however, have an astringent or bitter taste, and render conventional extracts of such plant sources unsuitable for use as sweetening agents.
It is the primary object of the present invention to provide a process for extracting diterpene glycosides wherein the resulting product has a useful sweet flavor profile.
It is a further object of the present invention to provide a process for extracting diterpene glycosides which provides a highly concentrated diterpene glycoside end product.
It is a still further object of the present invention to provide a process for extracting diterpene glycosides which uses minimal amounts of organic or inorganic chemicals so as to provide a high yield of diterpene glycosides in an efficient, simple, consistent and economic process.
Other objects and advantages of the present invention will appear hereinbelow.
SUMMARY OF THE INVENTION
In accordance with the present invention, the foregoing objects and advantages have been readily attained.
According to the invention, a process for obtaining diterpene glycosides has been provided, which process comprises the steps of providing a plant source material containing diterpene glycosides, obtaining a liquid extract from said source material, said liquid extract containing said diterpene glycosides; mixing said liquid extract with a solution saturated with at least one metallic ion selected from the group consisting of metallic ions having an oxidation number of three, metallic ions having an oxidation number of two, and combinations thereof so as to provide a mixture; allowing said mixture to rest so as to provide a solid precipitate material and a liquid portion containing soluble portions of said liquid extract including said diterpene glycosides;
passing said liquid portion through a first column containing a neutral absorptive macroporous resin so as to absorb portions of said liquid portion including said diterpene glycosides onto said neutral absorptive macroporous resin; rinsing said first column with an alcohol so as to obtain an alcohol solution containing said diterpene glycosides; passing said alcohol solution through a second column containing an alkaline macroporous resin so as to provide a purified alcohol solution containing said diterpene glycosides; and drying said purified alcohol solution so as to provide a dry composition containing said diterpene glycosides.
The process of the present invention provides a dry composition final product which contains a high yield of diterpene glycosides, as well as certain beneficial and non-bitter tannins, while astringent or bitter tasting tannins and other undesirable and/or insoluble materials are removed and disposed of so as to be substantially absent in the final product.
REFERENCES:
patent: 0727492 (1996-08-01), None
patent: 52-105260 (1977-09-01), None
patent: 58-28245 (1983-02-01), None
patent: 59-51261 (1984-12-01), None
patent: 1604838 (1990-11-01), None
Ulubelen et al., “Terpenoids fromSalvia Sclarea,” Phytochemistry, 36(4), 971-974 (Jul. 12, 1994).*
Ohtani et al. (I), “Labdane-Type Diterpene Glycosides from Fruits ofRubus foliolosus,” Chemical&Pharmaceutical Bulletin(Japan), 39(9), 2443-2445 (Sep., 1991).*
Hashimoto et al., “Effect of Capsianoside, a Diterpene Glycoside, on Tight-Junctional Permeability,”Biochimica et Biophysica Acta, 1323(2), 281-290 (Jan. 31, 1997).*
Ohtani et al. (II), “Minor Diterpene Glycosides for Sweet Leaves ofRubus Suavissimus,” Phytochemistry, 31(5), 1553-1559 (May 1, 1992).*
Budavari et al. (eds.),The Merck Index, 11th Edition, Merck & Co., Rahway, NJ, 1989, only p. 1387 supplied, see espically entry No. 8766 (Stevioside).*
Wood et al., “Stevioside. I. The Structure of the Glucose Moieties,”Journal of Organic Chemistry, 20, 875-883 (Jul., 1955).*
DuBois et al., “Diterpenoid Sweeteners. Synthesis and Sensory Evaluation of Stevioside Analogues Nondegradable to Steviol,”Journal of Medicinal Chemistry, 24(11), 1269-1271 (Nov., 1981).
He Weiping
Zhou James H.
Bachman & LaPointe P.C.
Crane L. E.
Geist Gary
LandOfFree
Process for extracting sweet diterpene glycosides does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for extracting sweet diterpene glycosides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for extracting sweet diterpene glycosides will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2508439