Process for recovering Cu,Zn-superoxide dismutase from yeast

Details Process for recovering Cu,Zn-superoxide dismutase from yeast Process for recovering Cu,Zn-superoxide dismutase from yeast

1980-05-13

1982-07-20

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435259, C12N 902

Patent

active

043406750

ABSTRACT:
Cu,Zn-superoxide dismutase is recovered from yeast by plasmolysis with a small amount of ether or any other water immiscible, organic solvent and subsequent autolysis in water at a temperature of 25.degree. to 50.degree. C. and pH 5 to 9, following which the precipitate is removed and the superoxide dismutase is purified and isolated from the residual liquid, in particular by chromatography on carboxymethyl cellulose at pH 4.7 to 5.5.
Cu,Zn-superoxide dismutase from Saccharomyces cerevisiae has the amino acid sequence: ##STR1## where Cys-57 and Cys-146 form a disulfide bond.

REFERENCES:
patent: 3758682 (1973-09-01), Huber et al.
Goscin et al. in Biochimica et Biophysica Acta, vol. 289 (1972) pp. 276-283.

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