Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
1995-01-18
2001-04-17
Sisson, Bradley L. (Department: 1655)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S024310, C536S027400
Reexamination Certificate
active
06218106
ABSTRACT:
FIELD OF THE INVENTION
This invention relates to restriction fragment length polymorphism pattern tests useful to genotype domesticated fowl for the major histocompatibility B-G loci. The invention also relates to the use of certain B-G polypeptides to impart immunity to or to control the susceptibility of domesticated fowl to various diseases.
BACKGROUND OF THE INVENTION
In domesticated fowl the major histocompatibility complex (MHC) which is associated with the regulation of immune recognition and immune response is called the
B
system. Resistance to Marek's disease is closely related to the domesticated fowl MHC. Resistance to other diseases, general fitness, and productivity also appear to be influenced to some extent by MHC haplotype.
MHC haplotyping of chickens is presently done by hemagglutination assay which relies on the production of specific antisera. The assay in itself is technically simple. However, the production of the antisera and the interpretation of the assays require a highly trained individual. The MHC haplotypes present in commercial strains of chickens are usually a trade secret known only to individual breeders. Isolation of cloned gene sequences from the
B
system provides a means of developing alternative methods for MHC haplotyping of birds and for determining the genotype at particular loci within the
B
system. The interpretation of results is generally simpler and more uniform since typing by restriction fragment length polymorphism patterns is no longer dependent upon alloantisera which often require selective absorptions with blood samples from genetically-defined animals to delineate haplotype specificity.
SUMMARY OF THE INVENTION
The
B
system of histocompatibility in domesticated fowl is known to contain three subregions which are identified as
B-F
,
B-G
and
B-L
.
B-F
,
B-G
and
B-L
are described as subregions because multiple genes of each type are present within the region of the
B
system. This invention includes cDNA clones encoding
B-G
antigens of the
B
system. MHC haplotyping is accomplished by use of novel probes provided by these clones to detect restriction fragment length polymorphism (RFLP) patterns typical for various
B-G
alleles present at the multiple loci within the B-G subregion.
Genetic recombination within the
B
system of the chicken is rare. For that reason, while the probes of this invention screen for the
B-G
genes, additional genes also of importance to disease resistance may be located in regions within and closely adjacent to the
B
system and genetically and physically linked to the
B-G
type. Other genes of mostly unknown function are located within the MHC as well.
REFERENCES:
patent: 4988617 (1991-01-01), Landegren et al.
Nucleic Acid Hybridisation, A Practical Approach, Eds. B.D. Hames and S.J. Higgins, IRL Press, Washington, D.C. (1985) (Table of Contents only).
City of Hope
Rothwell Figg Ernst & Manbeck p.c.
Sisson Bradley L.
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