Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
1998-06-18
2001-06-05
Duffy, Patricia A. (Department: 1645)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C530S822000, C536S023700
Reexamination Certificate
active
06242571
ABSTRACT:
BACKGROUND OF INVENTION
Anaplasmosis, a vector-borne rickettsial disease of cattle, sheep and goats is caused by three species;
Anaplasma marginale, Anaplasma centrale
and
Anaplasma ovis
. Clinical disease is characterized by anemia, weight loss, abortion and death. Survivors are lifelong carriers of the rickettsia. Eventual control of Anaplasma species infection will require both an effective vaccine and identification of carrier cattle, sheep or goats. Two possible methods for routine carrier identification are a nucleic acid probe for hybridization of infected blood or the detection of Anaplasma species-specific antibody is serum. Hybridization of DNA extracted from blood with an
Anaplasma marginale
-specific nucleic acid probe does not always detect known carriers, because of cyclic changes in rickettsemia levels. Carrier identification by antibody requires that infected animals never clear the rickettsia. Indefinite persistence of
Anaplasma marginale
in infected cattle has been documented. Current serological tests for anaplasmosis are not widely used, primarily because the error rate is high. One problem with current tests is false positive results caused by erythrocyte contamination of the
Anaplasma marginale
antigen used in the tests, and the presence of anti-erythrocyte antibody in the sera of some cattle.
Recently, progress has been made toward the characterization of a surface membrane protein of
Anaplasma marginale
for use in diagnosis (N. Tebele, T. C. McGuire, and G. H. Palmer, Infect. Immun. 59:3199-3204, 1991 and E. S. Visser, T. C. McGuire, G. H. Palmer, W. C. Davis, V. Shkap, E. Pipano, and D. P. Knowles, Jr., Infect. Immun. 60:5139-5144, 1992.). This protein, designated major surface protein 5 (MSP-5) and monoclonal antibody ANAF16C1 were shown to have utility when used together in the competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) format (Anderson, J. Immunol. Meth., 74:139-149, 1984) for the diagnosis of cattle, sheep and goats infected with
Anaplasma marginale, Anaplasma centrale
and
Anaplasma ovis
(E. S. Visser, T. C. McGuire, G. H. Palmer, W. C. Davis, V. Shkap, E. Pipano, and D. P. Knowles, Jr., Infect. Immun. 60:5139-5144, 1992.).
BRIEF SUMMARY OF THE INVENTION
Disclosed and claimed here is a CI-ELISA using monoclonal antibody ANAF16C1 and the corresponding protein,
Anaplasma marginale
major surface protein-5, bound by monoclonal antibody ANAF16C1 for the identification of cattle, sheep or goats persistently infected with
Anaplasma marginale, Anaplasma centrale
or
Anaplasma ovis
. This invention provides a means of identifying cattle, sheep, or goats that are persistently infected with
Anaplasma marginale, Anaplasma centrale
or
Anaplasma ovis
. This test is specific for Anaplasma species detection since the specificity of this CI-ELISA resides solely in monoclonal antibody ANAF16C1 and monoclonal antibody ANAF16C1 as been shown to specifically bind to only Anaplasma species MSP-5. Since MSP-5 is conserved in all known Anaplasma species, it is logical to assert that MSP-5 is conserved in all isolates of Anaplasma species.
REFERENCES:
patent: 4134792 (1979-01-01), Boguslaski et al.
N. Tebele, T. McGuire and G. Palmer, “Induction of Protective Immunity by UsingAnaplasma marginaleInitial Body Membranes,”Infection and Immunity59:3199-3204 (1991).
E. Visser, T. McGuire, G. Palmer, W.C. Davis, V. Shkap, E. Pipano and D.P. Knowles Jr., “TheAnaplasma marginale msp5Gene Encodes a 19-Kilodalton Protein Conserved in all Recognized Anaplasma Species,”Infection and Immunity60:5139-5144 (Dec. 1992).
J. Anderson, “Use of Monoclonal Antibody in a Blocking ELISA to Detect Group Specific Antibodies to Bluetongue Virus,”Journal of Immunological Methods74:139-149 (1984).
E. Harlow and D. Lane, “Antibodies: A Laboratory Manual,”Cold Spring Harbor Laboratory(N.Y.) pp. 342 and pp. 511-552 (1988).
G.H. Palmer, A.F. Barbet, A.J. Musoke, J.M. Katende, F. Rurangirwa, V. Shkap, E. Pipano, W.C. Davis and T. McGuire, “Recognition of Conserved Surface Protein Epitopes onAnaplasma CentraleandAnaplasma MarginaleIsolates from Israel, Kenya and the United States,”Int. Journal for Parasitology18(1):33-38 (1988).
Davis William C.
Knowles Donald P.
McElwain Terry F.
McGuire Travis C.
Palmer Guy H.
Connor Margaret A.
Duffy Patricia A.
Fado John D.
Silverstein M. Howard
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