Non-peptide peptidomimetics

Details Non-peptide peptidomimetics Non-peptide peptidomimetics

1998-08-13

2001-03-06

Tsang, Cecilia (Department: 1626)

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

C548S468000, C548S517000, C549S417000

Reexamination Certificate

active

06197963

ABSTRACT:

FIELD OF THE INVENTION
This invention relates to synthetic compounds which mimic or inhibit the biological and/or chemical activity of peptides, including compounds which bind G-protein-linked receptors, like the somatostatin (SRIF) receptors, and NK-1 receptors.
BACKGROUND OF THE INVENTION
Peptides are implicated in a wide variety of biochemical processes in humans and other mammals. For example, it is known that a number of hormones and neurotransmitters are controlled by receptor-mediated stimulation of one or more of a family of guanine nucleotide-binding regulatory proteins, known as G-proteins. G-proteins activate or inhibit different effector enzymes, modulating the levels of intracellular second messengers. At least 50 sub-types of G-protein-linked (G-protein coupled) receptors have been identified, among them the &agr;-adrenergic, &bgr;-adrenergic, muscarinic, cholinergic, dopamine, histamine, adenosine, serotonin, prostaglandin, leukotriene, thromboxane, prostacyclin, PAF, cAMP, enkephalin, endorphin, cholecystokinin, bombesin, substance K, substance P, neuromedin, bradykinin, FMLP, C5a, C3a, vasopressin, oxytocin, angiotensin, VIP, parathyroid hormone, calcitonin, neurotensin, TRH, somatostatin, rhodopsin, epinephrine, norepinephrine, acetylcholine, S-hydroxytryptamine, thyrotropin, thyrotropin releasing hormone, follicle stimulating, lutropin, choriogonadotropin, thrombin, retinal, and olfactory receptors. Nine or more G-proteins and at least seven effector systems have also been described. All of the G-protein-linked receptors analyzed to date contain from one to three potential sites of asparagine-linked glycosylation.
The transmembrane signaling pathway used by G-protein-linked receptors represents one of the major mechanism of signal transduction in cellular systems. It is known, for example, that substance P acts as a vasodilator, a depressant, stimulates salivation, and produces increased capillary permeability. Substance P is a naturally occurring undecapeptide belonging to the tachykinin family of peptides, the latter being so-named because of their prompt contractile action on extravascular smooth muscle tissue. In addition to substance P (neurokinin-1 receptor, NK-1), the known mammalian tachykinins include neurokinin A (NK-2 receptor) and neurokinin B (NK-3). The tachykinins have been implicated in gastrointestinal (GI) disorders and diseases of the GI tract, such as inflammatory bowel disease, ulcerative colitis and Crohn's disease.
Substance P is known to produce both analgesia and hyperalgesia in animals, depending on dose and pain responsiveness of the animal and plays a role in sensory transmission and pain perception. Substance P also is believed to be involved in the inflammatory response in diseases such as rheumatoid arthritis and osteoarthritis. Other disease areas where the tachykinins are believed to be involved include allergic conditions, immunoregulation, bronchospasm, reflex or neuronal control of the viscera, and Alzheimer's disease and Downs Syndrome.
Somatostatin, a cyclic tetradecapeptide, has attracted attention for its therapeutic potential. Brazeau et al., Science 1973, 179, 77-79. Brazeau et al.,
Can. J. Biochem.
1974, 52, 1067-1072; Rivier et al.,
J. Am. Chem. Soc.
1974, 96,2986-2992;] Hirschmann et al. “Some Recent Developments in the Chemistry and Biology of Somatostatin-Related Peptides”. In
Chemistry of Natural Products: The Proceedings of Sino
-
American Symposium on Chemistry of Natural Products
; Yu, W., Ed.; Gordon and Breach, Science Publishers: New York, 1982; pp 44-54 and references cited therein. Indeed the parenteral peptidal drug octreotide has been approved for clinical use; see: Bauer, W.; Briner, U.; Doepfner, W.; Haller, R.; Huguenin, R.; Marbach, P.; Petcher, T. J.; Pless, J.
Life Sciences
1982, 31, 1133-1140. Lamberts, S. W.; van der Lely, A.-J.; de Herder, W. W.; Hofland, L. J. Octreotide.
New Engl. J. Med.
1996, 334, 246-254. Sassolas, G.
Eur. J. Endocrinology
1995, 133, 675-677. It has been demonstrated that Phe
7
of somatostatin has an axial disposition.
To date, there have been limited therapeutic applications involving peptides, due in considerable part to lack of oral bioavailability and to proteolytic degradation. Typically, for example, peptides are rapidly degraded in vivo by exo- and endopeptidases, resulting in generally very short biological half-lives. Another deficiency of peptides as potential therapeutic agents is their lack of bioavailability via oral administration. Degradation of the peptides by proteolytic enzymes in the gastrointestinal tract is likely an important contributing factor. The problem is, however, more complicated, because it has been recognized that even small, cyclic peptides which are not subject to rapid metabolic inactivation nevertheless exhibit poor oral bioavailability. This likely is due to poor transport across the intestinal membrane and rapid clearance from the blood by hepatic extraction with subsequent excretion into the intestine. These observations suggest that multiple amide bonds may interfere with oral bioavailability.
The design of peptide mimics which are resistant to degradation by proteolytic enzymes has become of increasing interest to peptide chemists, both for hormone agonist/antagonist and for enzyme inhibitor design. A primary goal has been to reduce the susceptibility of mimics to cleavage and inactivation by peptidases. In one approach, such as disclosed by Sherman and Spatola,
J. Am. Chem. Soc.,
112, 1990, 433, one or more amide bonds have been replaced in an essentially isosteric manner by a variety of chemical functional groups. This stepwise approach has met with some success in that active analogs have been obtained. In some instances, these analogs have been shown to possess longer biological half-lives than their naturally-occurring counterparts. Nevertheless, this approach has limitations. Successful replacement of more than one amide bond has been rare. Consequently, the resulting analogs have remained susceptible to enzymatic inactivation elsewhere in the molecule. Moreover, this approach does not permit generalizations between chemically unrelated peptides concerning permissible amide mimic substitutions.
In another approach, a variety of uncoded or modified amino acids such as D-amino acids and N-methyl amino acids have been used to modify mammalian peptides. Alternatively, a presumed bioactive conformation has been stabilized by a covalent modification, such as cyclization or by incorporation of y-lactam or other types of bridges. See, e.g., Veber and Hirschmann, et al.,
Proc. Natl. Acad. Sci. USA,
1978 75 2636 and Thorsett, et al.,
Biochem Biophys. Res. Comm.,
1983, 111, 166. The primary purpose of such manipulations has not been to avoid metabolism or to enhance oral bioavailability but rather to constrain a bioactive conformation to enhance potency or to induce greater specificity for a receptor subtype.
Another approach, disclosed by Rich, D. H. in
Protease Inhibitors
, Barrett and Selveson, eds., Elsevier, p. 179-217 (1986), has been to design peptide mimics through the application of the transition state analog concept in enzyme inhibitor design. For example, it is known that the secondary alcohol of statine mimics the tetrahedral transition state of the scissile amide bond of the pepsin substrate. Again, increased potency rather than decreased susceptibility to peptidases or increased bioavailability was the principal objective. Moreover, the transition state analog concept has no apparent relevance to hormone agonist/antagonist design.
Nicolaou and Hirschmann, et al.,
Design and synthesis of a peptidomimetic employing &bgr;
-
D
-
glucose for scaffolding
, in Peptides, Chemistry, Structure and Biology: Proceedings of the 11th American Peptide Symposium, Rivier and Marshall, eds., ESCOM (1990), p. 881-884, disclosed non-peptide somatostatin mimics having structures (III-4a) and (III-5a), wherein Bn is benzyl.
These mimics bound somatostatin receptors of AtT-20 cells with IC
50
of about 15&t

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