Detecting and distinguishing between plasminogen activators

Details Detecting and distinguishing between plasminogen activators Detecting and distinguishing between plasminogen activators

1983-07-12

1986-12-16

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 13, 435215, 435217, G01N 3353, C12Q 156, C12N 172, C12N 168

Patent

active

046296948

ABSTRACT:
This invention relates to a method for determining fibrinolytic activation in human blood and to a method to distinguish between tissue (e.g. vascular) plasminogen activator and urokinase-like plasminogen activator contributions to fibrinolytic activation.
Broadly, the method of the invention relates to detecting the level of plasmin-plasmin inhibitor complexes in blood plasma samples before and after clotting thereof; a greater increase in the complex level in the clotted sample as compared to non-clotted samples indicating the presence of circulating tissue plasminogen activator, with substantially identical levels of increase of complex in both the clotted and unclotted samples indicating the sole presence of urokinase-type plasminogen activator in the sample.

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