Homogeneous specific binding assay method

Details Homogeneous specific binding assay method Homogeneous specific binding assay method

Filed on

1978-04-10

Date issued

1986-12-16

Examiner

Naff, David M.

Patent Class

Chemistry: molecular biology and microbiology

SubClass

Measuring or testing process involving enzymes or...

SubSubClass

Involving antigen-antibody binding, specific binding protein...

Other Related Categories

435174, 436537, 436544, 436546, G01N 3354, G01N 3358, C12N 1100

Type

Patent

Status

active

Patent number

046296883

Description

ABSTRACT:
A test composition, device, and method for their use in a homogeneous specific binding assay which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. The test composition and device comprise a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand. The effect of the specific binding reaction on the activity of the conjugated reactant is related to the presence or amount of the ligand in the liquid medium tested.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3839153 (1974-10-01), Schuurs et al.
patent: 3880934 (1975-04-01), Rammler
patent: 3998943 (1976-12-01), Ullman
Miles, et al., Labelled Antibodies and Immunological Assay Systems, Nature, vol. 29, 1968 (pp. 186-189).

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