Rnase-cv (coriolus versicolor)

Details Rnase-cv (coriolus versicolor) Rnase-cv (coriolus versicolor)

1994-12-19

1998-10-20

Tsang, Cecilia J.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

514 8, 514 12, 530322, 530324, 530327, 530350, 530371, 530395, 530523, 5361231, A61K 3800, C07K 1700

Patent

active

058246487

ABSTRACT:
A method of obtaining a novel polypeptide from a crude extraction product of polysaccharide peptide Coriolus versicolor comprising: a) boiling a water soluble powder of polysaccharide peptide Coriolus versicolor; b) centrifuging a boiled product from step a); c) filtering a centrifuged product from step b); d) purifying a solution from step c) by gel filtration chromatography; e) subjecting the purified material from step d) to HPLC using a reversed-phase at ambient temperature, f) subjecting the purified material from step e) to capillary isoelectrophoresis focusing; g) further purifying this product by HPLC and ionic exchange columns and h) purifying a protein by SDS-PAGE and i) recovering a peptide from 12 Kd to 16 kD. The peptide has the partial amino acid sequence GTAAAKEFERQHM SEQ ID NO:1.

REFERENCES:
patent: 4289688 (1981-09-01), Ha et al.
patent: 4820689 (1989-04-01), Ikuzawa et al.
patent: 5084160 (1992-01-01), Stewart et al.
patent: 5374714 (1994-12-01), Yang et al.
Yang et al, Am. J. Chain, Med., vol. 20, No. 3-4, pp. 221-232, 1992 (only abstract provided).
Yang et al., Am. J. Chin. Med., vol. 20, No. 3-4, pp. 221-232, 1992 (only Abstract provided).
Cho et al., Chemical Abstracts, vol. 110, p. 333, Ref. No. 228284x, 1989.

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