Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2000-02-24
2001-08-21
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S006120, C435S320100, C435S325000, C435S252300, C536S023200
Reexamination Certificate
active
06277612
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to isolated polypeptides having galactose oxidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
2. Description of the Related Art
Galactose oxidases (EC 1.1.3.9) catalyze the oxidation of D-galactose in the presence of molecular oxygen to D-galacto-hexodialdose and hydrogen peroxide.
Galactose oxidases have been isolated from
Polyporus circinatus
(Amarai et al., 1966,
Methods in Enzymology
9: 87-92; Avigad et al., 1962,
Journal of Biological Chemistry
237: 2736-2743; Kosman et al., 1974,
Archives of Biochemistry and Biophysics
165: 456-467),
Dactylium dendroides
(Tressel and Kosman, 1982,
Methods in Enzymology
89: 163-171; Kellener et al., 1988,
Archives of Biochemistry and Biophysics
262: 349-354),
Gibberella fujikuroi
(Asaka and Terada, 1982,
Agricultural and Biological Chemistry
46: 333-340),
Fusarium gram inearum
(Dias and Kernmelmeir, 1987,
Rev. Microbiol.
18: 276-278)
Genes encoding galactose oxidases have been isolated from
Stigmatella aurantiaca
(Silakowski et al., 1998,
Journal of Bacteriology
180: 1241-1247) and
Dactylium dendroides
(McPherson et al., 1992,
Journal of Biological Chemistry
267: 8146-8152).
It is an object of the present invention to provide improved polypeptides having galactose oxidase activity and nucleic acid encoding the polypeptides.
SUMMARY OF THE INVENTION
The present invention relates to isolated polypeptides having galactose oxidase activity selected from the group consisting of:
(a) a polypeptide having an amino acid sequence which has at least 65% identity with amino acids 21 to 679 of SEQ ID NO:2;
(b) a polypeptide encoded by a nucleic acid sequence which hybridizes under medium stringency conditions with (i) nucleotides 61 to 2037 of SEQ ID NO:1, (ii) the cDNA sequence contained in nucleotides 61 to 2037 of SEQ ID NO:1, (iii) a subsequence of (i) or (ii) of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii), or (iii);
(c) a variant of the polypeptide having an amino acid sequence of SEQ ID NO:2 comprising a substitution, deletion, and/or insertion of one or more amino acids;
(d) an allelic variant of (a) or (b); and
(e) a fragment of (a), (b), or (d) that has galactose oxidase activity.
The present invention also relates to isolated nucleic acid sequences encoding the polypeptides and to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
REFERENCES:
Amaral et al., 1966, Methods in Enzymology 9:87-92.
Avigad et al., 1962, Journal of Biological Chem 237:2736-2743.
Kosman et al., 1974, Archives of Biochemistry & Biophysics 165:456-467.
Tressel & Kosman, 1982, Methods in Enzymology 89:163-171.
Kelleher et al., 1988, Archives of Biochem. & Biophysics 263:349-354.
Aisaka & Terada, 1982, Agric. Biol. Chem. 46:1191-1197.
Dias & Kemmerlmeir, 1987, Rev. Microbiol. 18:276-278.
Silakowski et al., 1998, Journal of Bacteriology 180:1241-1247.
McPherson et al., 1992, Journal of Biological Chemistry 267:8146-8152.
Berka Randy M.
Golightly Elizabeth
Rey Michael W.
Monshipour M.
Novo Nordisk Biotech Inc.
Prouty Rebecca E.
Starnes Robert L.
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