Creatinine iminohydrolase free from urease activity

Details Creatinine iminohydrolase free from urease activity Creatinine iminohydrolase free from urease activity

1979-11-05

1981-06-30

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435227, 435850, C12Q 134, C12N 978

Patent

active

042763770

ABSTRACT:
A urease-free creatinine iminohydrolase enzyme preparation obtained from an aerobic soil microorganism. The enzyme of the preparation preferably has a molecular weight of from about 250,000 to 300,000; a maximum activity at a pH between 7 and 8 as measured at 37.degree. C.; a K.sub.m of about 3 to 5 mM for creatinine as measured at 37.degree. C., pH 7.5; and a specific activity for creatinine of at least about 1.0 unit per milligram of protein in the preparation as measured at 37.degree. C., pH 7.5. The preferred enzyme preparation is derived from the aerobic soil microorganism ATCC 31,546. Assay methods, compositions, and elements containing the aforementioned urease-free creatinine iminohydrolase for the determination of creatinine in an aqueous liquid are also disclosed.

REFERENCES:
patent: 3806420 (1974-04-01), Holz et al.
patent: 3907644 (1975-09-01), Mollering et al.
patent: 4039384 (1977-08-01), Suzuki et al.
patent: 4087329 (1978-05-01), Terada et al.
Szulmajster, Journal of Bacteriology, vol. 75, pp. 633-639 (1958).
Szulmajster, Biochem. Biophys. Acta, vol. 30, pp. 154-163 (1958).

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