Regenerable insoluble support for protein immobilization

Details Regenerable insoluble support for protein immobilization Regenerable insoluble support for protein immobilization

1979-06-12

1981-07-21

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

252411R, 260141, 426 34, 435178, 435181, C12N 1100, C12N 1116, C12N 1110, C12N 1106

Patent

active

042799988

ABSTRACT:
To permit regeneration of an insoluble support for immobilized proteins, the protein is connected to the support by a spacer arm containing a diazo linkage and when the protein is denatured, the spacer arm is broken by reducing the diazo linkage to remove the denatured protein. New native protein of the same or of a different type is joined to the arm to permit reuse of the support. A preferred support containing a spacer arm is p-(N-acetyl-L-tyrosine azo)benzamidoethyl agarose. A protein is coupled to the carboxyl group of N-acetyl-L-tyrosine and when the protein is spent, the azo bond is reduced to remove the protein and leave p-aminobenzamido agarose which is diazotized and recoupled to N-acetyl-L-tyrosine which is then coupled to fresh protein.

REFERENCES:
patent: 4043869 (1977-08-01), Barker et al.
Cuatrecasas, et al., Adsorbents For Affinity Chromatography, Biochemistry, vol. 11, No. 12, 1972 (pp. 2291-2298).
Cuatrecasas, et al., Affinity Chromatography, Methods of Enzymology, vol. XXII, 1971 (pp. 345-379).
Cuatrecasas, P., Protein Purification By Affinity Chromatography, J. Biol. Chem., vol. 245, No. 12, 1970 (pp. 3059-3065).
Woychik et al., Preparation and Application of Immobilized B--Galactosidase of Saccharomyces Lactis Immobilized Enzymes in Food and Microbial Processes, Plenum Press, N.Y., 1974 (pp. 41-49).

Affiliated with

Also associated with

Rating

Regenerable insoluble support for protein immobilization does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Regenerable insoluble support for protein immobilization, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Regenerable insoluble support for protein immobilization will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2433171