Glycosyltransferases for biosynthesis of oligosaccharides, and g

Details Glycosyltransferases for biosynthesis of oligosaccharides, and g Glycosyltransferases for biosynthesis of oligosaccharides, and g

1997-06-18

1999-08-31

Hobbs, Lisa

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

4352523, 43525411, 4353201, 435325, 536 232, 536 243, C12N 910, C12N 120, C12N 1500, C07H 2104

Patent

active

059453222

ABSTRACT:
The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase locus of Neisseria gonorrhoeae containing five open reading frames for five different glycosyltransferases. The functionally active glycosyltransferases of the invention are characterized by catalyzing reactions such as adding Gal .beta.1.fwdarw.4 to GlcNAc or Glc; adding GalNAc or GlcNAc .beta.1.fwdarw.3 to Gal; and adding Gal .alpha.1.fwdarw.4 to Gal. The glycosyltransferases of the invention are particularly suited to the synthesis of the oligosaccharides Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4Glc (a mimic of lacto-N-neotetraose), GalNAc.beta.1.fwdarw.3Gal.beta.1.fwdarw.4GlcNAc.beta.1.fwdarw.3Gal.beta.l. fwdarw.4Glc.beta.1.fwdarw.4 (a mimic ganglioside), and Gal.alpha.1.fwdarw.4Gal.beta.1.fwdarw.4Glc.beta.1.fwdarw.4Hep.fwdarw.R (a mimic of the saccharide portion of globo-glycolipids).

REFERENCES:
patent: 5180674 (1993-01-01), Roth
Sandlin & Stein, 1994, J. Bacteriol. 176:2930-2937.
Zhou et al., 1994, J. Biol. Chem. 269:11162-11169.
Gibson et al., 1993, J. Bacteriol. 175:2702-2712.
Jennings et al., 1993, Molec. Microbiol. 19:361-369.
High et al., 1993, Molec. Microbiol. 9:1275-1282.
Robertson et al., 1993, Molec. Microbiol. 8:891-901.
van Putten et al., 1993, EMBO J. 12:4043-4051.
Verheul et al., 1993, Microbiol, Rev. 57:34-49.
Yamasaki et al., 1993, J. Bacteriol. 175:4565-4568.
Kerwood et al., 1992, Biochem. 31:12760-12768.
Knight et al., 1992, Molec. Microbiol. 6:1565-1573.
Mandrell, 1992, Infect. Immunol. 60:3017-3020.
Pradel et al., 1992, J. Bacteriol. 174:4736-4745.
Cope et al., 1991, Molec. Microbiol. 5:1113-1124.
John et al., 1981, J. Biol. Chem. 266:19303-19311.
Jonsson et al., 1991, EMBO J. 10:477-488.
Mandrell et al., 1991, J. Bacteriol. 173:2823-2832.
Schneider et al., 1991, J. Exp. Med. 174:1601-1605.
Tsai & Civin, 1991, Infect. Immun. 59:3604-3609.
Mandrell et al., 1990, J. Exp. Med. 171:1649-1664.
Parsons et al., 1989, Microb. Pathog. 7:63-72.
Dudas & Apicella, 1988, Infect. Immun. 56:499-504.
Mandrell et al., 1988, J. Exp. Med. 168:107-126.
Schneider et al., 1988, Infect. Immun. 56:942-946.
Apicella et al., 1987, Infect. Immun. 55:1755-1761.
Webster et al., 1983, J. Biol. Chem. 258:10637-10641.
Gotschlich, 1994, J. Exp. Med, 180:2181-2190.
Stephens et al., 1994, Infect. & Immun. 2:2947-2952.
Zhou et al., 1994, Molec. Microbiol. 14:609-618.

Affiliated with

Also associated with

Rating

Glycosyltransferases for biosynthesis of oligosaccharides, and g does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Glycosyltransferases for biosynthesis of oligosaccharides, and g, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Glycosyltransferases for biosynthesis of oligosaccharides, and g will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2426378