Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;...
Patent
1997-12-31
1999-08-31
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
424422, 424 941, 435176, 435177, 435180, 435181, 436518, 436524, 436531, 436532, 530402, 530810, 530811, 530815, 530816, C12N 1100, G01N 33543, C07K 100, A61K 3843
Patent
active
059453192
ABSTRACT:
Methods are provided for forming a coating of an immobilized biomolecule on a surface of a medical device to impart improved biocompatibility for contacting tissue and bodily fluids. A 2-aminoalcohol moiety of a biomolecule is oxidized with a periodate to an aldehyde moiety which is reacted with an amine moiety on the surface of a medical device to form an imine moiety, and the imine moiety is reduced to form an amine linkage immobilizing a coating of the biomolecule on the surface. In another method, a biomolecule coating containing an amine moiety and a 2-aminoalcohol moiety is immobilized on the surface of a medical device, the 2-aminoalcohol moiety is oxidized with a periodate to an aldehyde moiety which is reacted with the amine moiety to form an imine moiety, and the imine moiety is reduced to form a secondary amine and crosslink the coating. Each biomolecule may contain an amine moiety and a 2-aminoalcohol moiety, and the aldehyde moiety of one biomolecule resulting from periodate oxidation may react with the amine moiety of another biomolecule. In a further embodiment, a 2-aminoalcohol moiety on the surface of a medical device is oxidized with a periodate to an aldehyde moiety, and an amine moiety of a biomolecule is reacted with the aldehyde moiety to form an imine moiety which is reduced to form an amine linkage immobilizing the biomolecule on the surface.
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Medtronic Inc.
Naff David M.
Patton Harold R.
Woods Thomas F.
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