Large scale DNA microsequencing device

Details Large scale DNA microsequencing device Large scale DNA microsequencing device

1997-04-23

1999-08-31

Warden, Robert

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204466, 204606, 204616, 435 6, 4352872, G01N 2726, G01N 27447, C12Q 168

Patent

active

059449713

ABSTRACT:
A microminiature sequencing apparatus and method provide means for simultaneously obtaining sequences of plural polynucleotide strands. The apparatus comprises a microchip into which plural channels have been etched using standard lithographic procedures and chemical wet etching. The channels include a reaction well and a separating section. Enclosing the channels is accomplished by bonding a transparent cover plate over the apparatus. A first oligonucleotide strand is chemically affixed to the apparatus through an alkyl chain. Subsequent nucleotides are selected by complementary base pair bonding. A target nucleotide strand is used to produce a family of labelled sequencing strands in each channel which are separated in the separating section. During or following separation the sequences are determined using appropriate detection means.

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Micromachining a Miniaturized Capillary electrophoresis-Based chemical Analysis System on a Chip (Science Reports .circle-solid. vol. 261.circle-solid. Aug. 13, 1993; pp. 895-897).

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