Process for the continuous production of L-carnitine

Details Process for the continuous production of L-carnitine Process for the continuous production of L-carnitine

1985-03-29

1987-11-24

Warren, Charles F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing nitrogen-containing organic compound

435106, 435287, 435824, 435825, C12P 1300, C12P 1301

Patent

active

047089369

ABSTRACT:
Process for the continuous production of L-carnitine by the microbiological method. A microorganism of the strain DSM No. 3225 (HK 1331b) type is cultivated in a bioreactor with crotonobetaine and/or .gamma.-butyrobetaine in the presence of a growth substrate. The culture fluid passes outside of the bioreactor in a circulation in which a separation of the cell is carried out. A quantity of cell-free solution, which is as large as the amount fed to the bioreactor as a substrate, is withdrawn from the bioreactor. The L-carnitine is separated from the cell-free solution.

REFERENCES:
patent: 4371618 (1983-02-01), Cavazza
Ornston et al., Biochemical and Biophysical Research Communications, vol. 36, No. 1, (1969), pp. 179-184.
Perry's Chemical Engineers Handbook, 6th Edition, (1984), pp. 27-7.
J. H. Miller, "Experiments in Molecular Genetics", Cold Spring Laboratory, (1972), pp. 121 to 143, Experiments 13 through 17.
P. Gerhardt et al., (eds.), Manual of Methods for General Bacteriology, Am. Soc. for Microbiology, (1981), pp. 222 to 242.
Kulla et al., Arch. Microbiol. 135, 1 (1983), pp. 1 to 7.
J. P. Vandecasteele, Appl. Environ, Microbiol. 39, pp. 327 to 334, (1980).
Lindstedt et al., Biochemistry 6, pp. 1262-1270 (1967).
Lindstedt et al., Biochemistry 16, pp. 2181-2188 (1977).

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