Method of isolating albumin from blood products

Details Method of isolating albumin from blood products Method of isolating albumin from blood products

1976-09-28

1978-04-25

Adee, John

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

210DIG23, 210 31C, B01D 1508, A 2D 100

Patent

active

040862224

ABSTRACT:
This invention relates to a new method of isolating highly purified albumin in high yields from plasma products.
According to the invention a particular plasma fraction, containing albumin in dissolved form and being essentially free from the coagulation factors I, II, VII, VIII, IX and X and from the main part of IgG, is subjected to a two-step chromatographic separation using aqueous buffer systems. The first step is carried out on an anion exchanger and the second step on a cation exchanger, or vice versa. In a preferred embodiment a buffer of pH 4.5 - 4.9 and an ionic strength of 0.025 - 0.1 is used for the separation on the anion exchanger, whereas a buffer of pH 5.2 - 6.5 and an ionic strength of 0.1 - 0.05 is used for the separation on the cation exchanger.

REFERENCES:
patent: 3657116 (1972-04-01), Haller
patent: 3959128 (1976-05-01), Harris
Separation of Neutral Proteins on Ion-Exchange Resins by Boardman et al. in Biochemical Journal, vol. 59, No. 4, Apr. 1955, pp. 543, 552.

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