Method for aggregating cells with small microspheres

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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43524025, 435174, 435176, 435177, 435178, 435180, C12N 500, C12N 502, C12N 1100

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active

051148552

ABSTRACT:
A method of inducing aggregate formation of animal cells is carried out by forming a nutrient medium suspension of animal cells and introducing itno the suspension microspheres of a derivatized diameter of not more than about 60 .mu.m, when measured in a suitable buffer saline or cell culture media.

REFERENCES:
patent: 4266032 (1981-05-01), Miller et al.
H. Kubota, Cell Culture Using Microcarrier: The Effects of Chemical and Physical Properties of Microcarrier on Cell Attachment, Spreading and Growth (JAACT Meeting).
Hu, W. S. & Wang, D. I. C. (1987) Biotechnology & Bioengineering 30:548-557.
Kubota, H. & Nagaike (1989) Abstract, Japanese Assn of Animal Cell Technology Annual Meeting, Tsukuba City, Japan Nov. 20-22, 1989.
Fiorentine, D., Shahar, A. and Mizrahi, A. (1985), Production of Herpesvirrus of Turkeys In Microcarrier Culturing System--A New Method For Production of Vaccine Against Marek's Disease, Develp. Biol. Standard 60:421-430.
Lazar, A., Silverstein, L. and Mizahri, A. (1985), Agarose-Polyacrolein Microsphere Beads: A new Microcarrier Culturing System, Develop. Biol. Standard, 60:457-465.
Kotler, M., Reuveny, S., Mizrah, A. and Shahar, A. (1985), Ion Exchange Capacity of DEAE Microcarriers Determined The Growth Pattern of Cells In culture, Develop. Biol. Standard, 60:225-261.
Avgerinos, G. C., Drapeau, D., Socolow, J. S., Mao, J., Hsiao, K., Broeze, R. J. (1990), Spin Filter Perfusion System For High Density Cell Culture-Production of Recombinant Urinary Type Plasminogen Activator In CHO Cells, Biotechnology 8:54-57.
SoloHill Engineering, Inc. (U.S.A. 1985), Bioglas Microcarrier Beads Price List and Promotional Materials (4 pages).
Percell Biolytical (Sweden, 1988), Technical Bulletin regarding Cultispher-G Macroporous Gelatin Microcarrier (4 pages).
Tung, A. S., Sample, J. vG., Brown, T. A., Ray, N. G., Hayman, E. G. and Runstadler, P. R. Jr. (1988), TPA Production Through Mass Culturing of Chinese Hamster Ovary Cells, Biopharm Manufacturing reprint.
Litwin, J. (1985), The Growth Of Human Diploid Fibroblasts As Aggregages With Cellulose Fibres In Suspension, Develop. Biol. Standard, 60:237-242.
Avgerinos, G. C., and Drapeau, D. (1988) Production Scale Mammalian Cell Suspension Perfusion Culture With A Rotating Wire Mesh Screen, abstract and presentation at the Engineering Foundation on Cell Culture Engineering, Palm Coast, Fla., U.S.A., Jan. 31-Feb. 5, 1988.
Kakare, S. B., Webster, J., and Tajiri, D. (1988), Modeling Of Continuous Culture of "Clumped" CHO Cells For The Production of Erythropoietin, abstract and presentation at the Engineering Foundation on Cell Culture Engineering, Palm Coast, Fla., U.S.A., Jan. 31-Feb. 5, 1988.
Reuveny, S. (N.Y., U.S.A. 1983), Microcarriers For Culturing Mammalian Cells And Their Applications, Advances in Biotechnological Processes 2, pp. 1-32.
Ventrex Laboratories, Inc. (Me., U.S.A. 1986), Promotional Materials for Ventreglas.RTM. glass beads.
Hu, W. S. and Wang, D. I. C. (1986), "Mammalian Cell Culture Technology: A Review From An Engineering Perspective," in Mammalian Cell Technology, Ed. W. G. Thilly, Butterworths Publishing Company pp. 167-197.
van Wezel, A. L. (1967), Growth of Cell-strains and Primary Cells on Micro-carriers in Homogenous Culture, Nature 216:64-65.
Johansson A. and Nielsen, V. (1980), Biosilon.RTM. A New Microcarrier, Develop. Biol. Standard, 46:125-129.
Kuo, M. J., Lewis, C. Jr., Martin, R. A., Miller, R. E. Schoenfeld, R. A., Schuck, J. M. and Wildi, B. S. (1981), Growth of Anchorage Dependent Mammalian Cells on Glycine-Derivatized Polystyrene in Suspension Culture, in vitro, vol. 17, No. 10, pp. 901-906.
Reuveny, S., Silberstein, L., Shahar, A., Freeman, E., and Mizrahi, A. (1982), Cell and Virus Propagation on Cylindrical Cellulose Based Microcarriers, Develop. Biol. Standard, 50:115-123.
Knazek, R. A., Gullino, P. M., Kohler, P. O., and Dedrick, R. L. (1972) Cell Culture on Artificial Capillaries: An Approach to Tissue Growth in vitro, Science 178:65-67.
Lydersen, B. K., Pugh, G. G., Paris, M. S., Sharma, B. P., Noll, L. A. (1985), Ceramic Matrix for Large Scale Animal Cell Culture, Biotechnical, Jan. 1985:63-67.
Hu, W. S., Meier, J., Wang, D. I. C. (1985), A Mechanistic Analysis of the Inoculum Requirement for the Cultivation of Mammalian Cells on Microcarriers, Biotechnology and Bioengineering 27:585-595.
Varni, J., Dame, J., Rediske, J., Beals, T. F., Hillegas, W. (1985), Substrate-Dependent Differences In Growth And Biological Properties of Fibroblasts And Epithelial Cells Grown In Microcarrier Culture, Journal of Biological Standardization 13:67-76.
Fisher, P. E. and Tickle, C. (1981), Differences In Alignment Of Normal And Transformed Cells On Glass Fibres, Exp. Cell Res. 131:Res. 131:407-410.
Tolbert, W. R., Hitt, M. M. and Feder, J. (1980), Cell Aggregate Suspension Culture For Large-Scale Production Of Biomolecules, in vitro 16(6):486-490.
Levine, D. W., Wang, D. I. C. and Thilly, W. G. (1979), Optimization of Growth Surface Parameters In Microcarrier Cell Culture, Biotechnology and Bioengineering 21:821-845.
Hu, W. S. and Dodge, T. C. (1985), Cultivation of Mammalian Cells in Bioreactors, Biotechnology Progress 1:209-215.
Blasey, H. D., Grossebueter, W., Lehman, J., Giehring, H. and Schwengers, D. (1988), B-interferon Production In Serum-free Medium on a New Type of Microcarrier, presentation at the Engineering Foundation on Cell Culture Engineering, Palm Coast, Fla., U.S.A., Jan. 31-Feb. 5, 1988.
Murata, M., Eto, Y. and Shibai, H. (1988), Large-scale Production of Erythroid Differentiation Factor By Gene-engineered Chinese Hamster Ovary (CHO) Cells In Suspension Culture, J. Ferment. Technol. 66:501-507.

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