Automatic focusing of biomedical specimens apparatus

Details Automatic focusing of biomedical specimens apparatus Automatic focusing of biomedical specimens apparatus

1994-09-20

1997-07-08

Mancuso, Joseph

Image analysis

Image enhancement or restoration

Focus measuring or adjusting

2502013, 348349, G06T 520

Patent

active

056470258

ABSTRACT:
A method for automatically focusing on a slide includes the steps of locating a coverslip, acquiring images from predetermined focal depths in the slide, and starting at an initial focal depth proximate the surface of the coverslip. A set of predetermined characteristics within each of the images are measured to generate at least one image measurement for each of the plurality of images. A focus measure is computed for each of the images, where each focus measure is a function of at least one image measurement. A best focus location is determined relative to a focal depth where an acquired image has a highest focus measure.

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