Mass propagation through shoot primordia and regeneration of pla

Details Mass propagation through shoot primordia and regeneration of pla Mass propagation through shoot primordia and regeneration of pla

1992-07-22

1994-05-10

Fox, David T.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524045, 43524049, 43524054, C12N 502, C12N 500

Patent

active

053106733

ABSTRACT:
A process for the mass propagation of a woody plant comprising the steps of removing a shoot tip from the woody plant, transplanting the shoot tip to an artificial medium containing inorganic salts and plant growth hormones as main ingredients, and rotary-culturing the shoot tip under illumination to form shoot primordia, and stationary-culturing the shoot primordia in a liquid medium to regenerate shoots. Further, a process for the regeneration of plants comprising the steps of preparing a shoot primordium by rotary-culturing a shoot tip of a plant, treating the shoot primordium with at least one enzyme to prepare a protoplast, culturing the protoplast in an artificial medium containing plant hormones and inorganic and organic salts as main ingredients to form a callus, and incubating the callus in a regeneration medium to regenerate a shoot; plus intermediate materials of the process, and processes for production of the intermediate materials.

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