Method for detection of antigens

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

424 8, 424 12, 435 34, C12Q 166

Patent

active

042581303

ABSTRACT:
False positive reactions with fluorescent antibodies such as Lactobacillus reaction with IgG-antiSalmonella are avoided by enzymatic treatment of the IgG to remove the non-antibody, Fc, fragment of the IgG.

REFERENCES:
patent: 3654090 (1972-04-01), Schurs
patent: 3966898 (1976-06-01), Sjoguist et al.
patent: 3992516 (1976-11-01), Lim
patent: 4002532 (1977-01-01), Weltman
patent: 4067959 (1978-01-01), Bolz
Reamer, et al., "Increased Sensitivity of Immunofluorescent Assay for Salmonella in Nonfat Dry Milk", Applied Microbiology, vol. 18, No. 3, (1969), pp. 328-331.
Thomason, et al., "Evaluation of Commercial Conjugates for Fluorescent Antibody Defection of Salmonellae", Applied Microbiology, vol. 27, No. 5, (1974), pp. 862-869.
Silliker et al., "The Fluorescent Antibody Technique as a Means of Detecting Salmonellae in Foods", J. Food Science, vol. 31, No. 1 (1966), pp. 240-244.
Tijssen et al., "Basic Techniques in Advanced Immunocyrochemistry Use of Enzymically Active Fab Fragments as Tracers", Chem. Abst., vol. 83, No. 3, p. 228 (1975), abs. No. 24530n.
Kabat, Structural Concepts in Immunology and Immunochemistry, Holt, Rinehart and Winston, Inc., New York (1968), pp. 162-167.
Gonatas et al., "I. Demonstration of Intracellular Immunoglobulin with .sup.125 I Fab Antibody Fragments II Segregation and Internalization of Immunoglobulin and of Lactoperoxidase-Iodnated Proteins of Lymphoid Cell Plasma Membranes", Immunoenzymatic Techniques, American Elsevier Pub. Co., New York, (1976), pp. 231-245.
Tharrington et al., "Nonspecific Staining of a Lactobacillus by Salmonella Fluorescent Antibodies", J. Food Science, vol. 43 (1978), pp. 548-552.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for detection of antigens does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for detection of antigens, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detection of antigens will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2408674

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.