Hybrid vector and method resulting in protein overproduction by

Details Hybrid vector and method resulting in protein overproduction by Hybrid vector and method resulting in protein overproduction by

1994-01-19

1997-07-08

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4353201, 435325, 435367, 435358, 435359, 435360, 435366, 4353723, 435372, 435362, C12P 2106, C12N 500, C12N 1563

Patent

active

056460090

ABSTRACT:
A hybrid vector carrying a first and second DNA segments operationally linked thereto, the first DNA segment encoding a protein capable of cross-linking to the cap structure of mRNA and mediating ribosome-binding, and the second DNA segment encoding a polypeptide or protein, the vector being capable of replication, transcription and translation to express the factor and the polypeptide or protein upon transformation of a eukaryotic host, and the polypeptide or protein being expressed at a level higher than the level of expression thereof in the absence of the first DNA segment. A eukaryotic host is transformed with this hybrid vector. Also disclosed is a method of increasing the synthesis of a polypeptide or protein in a eukaryotic host cell.

REFERENCES:
DeBenedetti et al., "Mammalian Expression Vectors for the in vivo study of eIF-4E", Abstr. Meeting on Translational Control, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, Sep. 1989.
DeBenedetti et al., "Over-Expression of eIF-4E, From an Episomal Vector in HeLa Cells results in Abnormal Growth and Ultimately Cell Death," Abstr. 2314, p. A2093, FASEB J. (1990).
Lazaris-Karatzas et al., "Malignant Transformation by a Eukaryotic Initiation Factor Subunit that Binds to mRNA 5" Cap", Nature, 345,544, (1990).
M. Kozak, "Selection of Translational Start Sites in Eukaryotic mRNAs," Current Communications in Molecular Biology, Cold Spring Harbor Laboratory, pp. 35-41, 1986.
De Benedetti, A. and R.E. Rhoads, "Overexpression of eukaryotic protein synthesis initiation factor 4E in HeLa cells results in aberrant growth and morphology," Proceedings of the National Academy of Science, vol. 87, pp. 8212-8216, Nov. 1990.
Joshi-Barve et al., "Alteration of the Major Phosphorylation Site of Eukaryotic Protein Synthesis Initiation Factor 4E Prevents its Association with the 48 S Initiation Complex," The Journal of Biological Chemistry, vol. 265, No. 5, pp. 2979-2983, (1990).
Hiremath et al., "In Vitro Synthesis, Phosphorylation, and Localization on 48S Initiation Complexes of Human Protein Synthesis Initiation Factor 4E," The Journal of Biological Chemistry, vol. 264, No. 2, pp. 1132-1138, (1990).
Rhoads, Robert E., "Cap recognition and the entry of mRNA into the protein synthesis initiation cycle," TIBS 13, Feb. 1988, pp. 52-56.
Fagan et al., "Translational Control of Ornithine Aminotransferase, Modulation by Initiation Factor eIF-4E," The Journal of Biological Chemistry, vol. 266, No. 25, pp. 16518-16523, (1991).
Koromilas et al., "mRNAs containing extensive secondary structure in their 5' non-coding region translate efficiently in cells overexpressing initiation factor eIF-4E," The EM80 Journal, vol. 11, No. 11, pp. 4153-4158, (1992).
Rychlik et al., "Amino acid sequence of the mRNA cap-binding protein from human tissues," Proceedings of the National Academy of Science, vol. S4, pp. 945-949, Feb. 1987.
De Benedetti, A. and R.E. Rhoads, "A novel BK virus-based episomal vector for expression of foreign genes in mammalian cells," Nucleic Acids Research, vol. 19, No. 8, pp. 1925-1931, 1991.
Shatzman, A.R. and M. Rosenberg, "[69]Expression, Identification, and Characterization of Recombinant Gene Products in Escherichia coli,"Methods in Enzymology, vol. 152, Academic Press, Inc., 1987, pp. 661-673.
Sambrook et al., Molecular Cloning: A Laboratory Manual, 1989, pp. 16.3-16.72.
Sarkar et al., Biochimica et Biophysica Acta, 1984, vol. 783: pp. 122-129.

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