Collection tip for fractionating solution gradients

Liquid purification or separation – With alarm – indicator – register – recorder – signal or... – Transparent

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210136, 210789, 356246, 422 72, 422100, 422101, 422103, B01D 1712

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active

056457154

ABSTRACT:
A fractionation apparatus has a displaceable piston operably mounted for displacing liquids from a centrifuge tube as the piston is inserted therein. The fractionation apparatus has a collection tip which has a seal for sealingly engaging an inner wall of the tube and has an axially extending passageway for passing liquids from within the tube to a collector as the piston is inserted into the tube. The passageway is generally a trumpet shape having a wide end and a narrow end. The wide end is presented to the liquids and the narrow end connects to the collector. The trumpet shape enhances laminar flow through the collection tip and reduced unwanted mixing between layers during fractionation.

REFERENCES:
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patent: 4346608 (1982-08-01), Olenick et al.
patent: 4464254 (1984-08-01), Dojki et al.
patent: 4753892 (1988-06-01), Coombs
patent: 5171539 (1992-12-01), Coombs
patent: 5266273 (1993-11-01), Coombs
Albright, J.F., Anderson, N.G.: "A Method of Rapid Fractionation of Particulate Systems By Gradient Differential Centrifugation", Experimental Cell Research 15, pp. 271-281 (1958).
Anderson, N.G., Bond, H.E., Canning, R.E.: "Analytical Techniques for Cell Fractions: I. Simplified Gradient Elution Programming", Analytical Biochemistry 3, pp. 472-478 (1962).
Candler, E.L., Nunley, C.E., Anderson, N.G.: "Analytical Techniques for Cell Fractions VI. Multiple Gradient-Distributing Rotor (B-XXI)", Analytical Biochemistry 21, pp. 253-258 (1967).
Anderson, N.G., Rutenberg, E.: "Analytical Techniques for Cell Fractions: VII. A Simple Gradient-Forming Apparatus", Analytical Biochemistry 21, pp. 259-265 (1967).
Coombs, D.: "Density Gradient Fractionation by Piston Displacement", Analytical Biochemistry 68, pp. 95-101 (1975).
"Model 139 Gradient Fractionator and Model 150 Motor Controller, Owner's Manual", Biocomp Instruments, Inc., Fredericton, Canada (1995).

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