Estrogen-progesterone control reagents and methods for making sa

Details Estrogen-progesterone control reagents and methods for making sa Estrogen-progesterone control reagents and methods for making sa

1983-06-29

1987-05-05

Kyle, Deborah L.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Preservative, buffer, anticoagulant or diluent

435 4, G01N 3100, C12Q 100

Patent

active

046632956

ABSTRACT:
Reagents useful for steroid receptor assays containing both progesterone and estrogen receptors and methods. The reagents of the instant invention contain both estrogen and progesterone receptors in a stabilized format. A preferred embodiment includes target tissue material obtained from calf uteri having the required receptors, HEPES suspending buffer, a binding site activation inhibitor, dithiothreotol, plexiform stabilizing matrix means and an amount of inactive protein added as necessary to meet predetermined total protein levels.

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Proksch et al., "Preparation of Lyophilized Abnormal Hemoglobin Controls for a Cellulose Acetate Electrophoresis", Brief Scientific Reports, vol. 74, No. 1:64-67.
Bonderman et al., "Lyophilized Control for Determination of Glucose-6-Phosphate Dehydrogenase Activity in Homolysates", Clin. Chem., vol. 25, 5:815-816 (1979).
Bonderman et al., "A Lyophilized Hemoglobin Control Prepared from Stroma Free Homolysates", Clin. Chem. 26/2:305-308 (1980).

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