Polynucleotide amplification mycoplasma assay, primers, and kits

Details Polynucleotide amplification mycoplasma assay, primers, and kits Polynucleotide amplification mycoplasma assay, primers, and kits

1993-11-23

1996-02-13

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435912, 536 2432, 536 2433, 935 17, 935 78, C12Q 168, C07H 2104, C12P 1934

Patent

active

054910625

ABSTRACT:
The invention described herein consists of methods and materials for the detection of mycoplasma infections. Mycoplasma infections may be detected in cell cultures and in animals. The subject methods use polynucleotide primer pairs that are capable of hybridizing to mycoplasma tRNA genes so as to provide for the generation of a distinctive set of amplification products when the primers are used in a cyclic amplification synthesis reaction, such as PCR (polymerase chain reaction). In addition to detecting mycoplasma infections, the subject methods may be used to identify the particular species of mycoplasma causing the infection. The subject invention also provides for primers and kits for performing the subject methods.

REFERENCES:
Van Kuppeveld, Applied and Environmental Biology (1992, Aug.) 58:2606-2615.

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