Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Metal containing – e.g. – chromoproteins – ferritin,...
Patent
1995-02-03
1997-04-08
Weimar, Elizabeth C.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Metal containing, e.g., chromoproteins, ferritin,...
530350, 530412, 530414, 530415, 530417, 530418, 530427, C07K 100, A23J 100
Patent
active
056189257
ABSTRACT:
The present invention relates to shark cartilage extracts and to a method of producing the same, these extracts having anti-angiogenic properties (reduction of the area of blood vessels observed in vivo on experimentally induced tumors), tumor regressive activity in vivo as well as demonstrating a direct inhibitory effect on tumor cell lines. This process does not involve any denaturing solvent or product and does not involve the use of any enzymes. It consists of obtaining a blend of whole cartilage in an aqueous solution of neutral pH, preferably pure water, this blend being centrifuged and the pellet and supernatant kept for further processing. The pellet is lyophylized and tested for anti-tumor and anti-angiogenic activities in vivo and in vitro, with or without supernatant. The supernatant has been shown to have anti-angiogenic and tumor regressive activities in vivo. The composition of the supernatant has then been investigated by different ways. Fractionation of this supernatant led to the characterization of some of its active components. The fractions were tested for their direct in vitro activity on cancer cell lines. Therefore, it is assumed that the non-fractionated supernatant has such an in vitro activity. Lyophilization substantially destroys the activity of these liquid fractions while no such abolition is observed in the solid extract.
REFERENCES:
patent: 3478146 (1969-11-01), Balassa
patent: 4042457 (1977-08-01), Kuettner et al.
patent: 4243582 (1981-01-01), Spilburg et al.
patent: 4350682 (1982-09-01), Balassa
patent: 4356261 (1982-10-01), Kuettner
patent: 4473551 (1984-09-01), Schinitsky
patent: 4656137 (1987-04-01), Balassa
patent: 4746729 (1988-05-01), Kuettner et al.
patent: 4749522 (1988-06-01), Kamarei
patent: 4822607 (1989-04-01), Balassa et al.
patent: 5075112 (1991-12-01), Lane
Moses et al., "Inhibitors of Angiogenesis", Biotechnology (1991) vol. 8, pp. 630-634.
Suzuki et al., "Cartilage-derived Antitumor Factor (CATF): A High Molecular Weight Fraction in Cartilage Extract Inhibits Solid Tumor Growth", Journal of Bone and Mineral Metabolism, 1984, vol. 2(3), pp. 3-7.
Luer et al., "Inhibitors of Angiogenesis from Shark Cartilage", 1986, Fed. Proc., vol. 45(4), p. 949 Abstract 4624.
Folkman and Klagsburn. (1987). Science. 235: 442.
Langer et al. (1976). Science. 193: 70.
Lee and Langer. (1983). Science. 221:1185-1187.
Oikawa et al. (1990). Cancer Letters. 51: 181-186.
Brazeau Paul
Dupont Eric
Juneau Christi
Les Laboratories Aeterna Inc.
Mohamed Abdel A.
Weimar Elizabeth C.
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