Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1986-12-08
1988-09-27
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 94, 435803, 435911, 127 461, 127 462, 127 58, 127 61, C12P 1902, C12P 1924, C12R 1645
Patent
active
047741830
ABSTRACT:
A low cost process for producing concentrated fructose is described which involves selective, preferential biological utilization of glucose in mixtures of glucose and fructose, in order to thereby yield concentrated fructose. Broadly speaking, the method involves contacting a mixture containing respective amounts of glucose and fructose with a microorganism which preferentially utilizes glucose as compared with fructose, incubating the mixture until the relative concentration of fructose is substantially in excess of that of glucose, and recovering concentrated fructose. In preferred forms, the microorganism is Pullularia pullulans, and the starting material may be a mixture of gluctose and fructose, sucrose, a carbohydrate such as inulin or starch; in the latter instances, the P. pullulans, by virtue of excretion of invertase, acts to degrade the starting material to give the desired glucose-fructose mixture. Recovery procedures such as ion exchange, evaporation and/or crystallization give highly concentrated or essentially pure fructose. A biopolymer side product resulting from glucose utilization by the preferred microorganism can also be recovered if desired.
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patent: 4276379 (1981-06-01), Heady
Bergey's Manual of Determinative Bacteriology, 8th Ed. Buchanan et al. (Eds.), The Williams and Wilkins Co., Baltimore (1974), pp. 512-566.
The Yeasts, Lodder (Ed.), North-Holland Publishing Co., London (1970), p. 1309.
Shipman, R. H. and Fan, L. T., "Bio-Plastics and SCP from Starch and Agricultural Wastes, vol. 13, #3, Process Biochemistry 1978, pp. 29-21.
Strandberg, G. W. and Smiley, K. L., "Free and Immobilized Glucose Isomerase from Streptomyecs Phaechromogenes", vol. 21, #4, Applied Microbiology, 1971, pp. 588-593.
Whitesides, G. M. and Wong, C. H., "Enzymes as Catalysts in Organic Synthesis, vol. 16, #2, Aldrichimica Acts 1983, pp. 27-34.
Kansas State University Research Foundation
Kepplinger Esther M.
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