Method of eliminating genetic routes for bacteriophage evolution

Details Method of eliminating genetic routes for bacteriophage evolution Method of eliminating genetic routes for bacteriophage evolution

1995-04-18

1997-04-08

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4352529, C12N 120, C12N 121

Patent

active

056187238

ABSTRACT:
A process of identifying and disrupting bacterial DNA sequences that contribute to the evolution of new lytic bacteriophages is described. Vectors and recombinant bacteria for use in producing fermentative starter cultures and culture resistant to the appearance of new phages, and methods of producing such vectors and recombinant bacteria, are described.

REFERENCES:
patent: 4883756 (1989-11-01), Klaenhammer et al.
patent: 5139950 (1992-08-01), Klaenhammer et al.
R.K. Thunell et al., Phage-Insensitive, Multiple-Strain Approach to Cheddar Cheese Making.sup.a, J. Dairy Science 64, pp. 2270-2277 (1981).
T.R. Klaenhammer, Genetic Characterization of Multiple Mechanisms of Phage Defense from a Prototype Phage-Insensitive Strain, Lactococcus iactis ME2.sup.1, J. Dairy Science 72, pp. 3429-3443 (1989).
Colin Hill, Bacteriophage and bacteriophage resistance in lactic acid bacteria, FEMS Microbiology Reviews 12, pp. 87-108 (1993).

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