Lifetime-resolved assay procedures

Details Lifetime-resolved assay procedures Lifetime-resolved assay procedures

1985-05-28

1989-04-18

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, 436537, 436546, 436800, 436805, C01N 33542, G12Q 168

Patent

active

048227331

ABSTRACT:
Improved luminescent lifetime-resolved association assay techniques for detection of analytes in samples using two photophore-labelled probes, the photophores of which have different emissive lifetimes. One of the photophores is excitable by a modulated energy source to an excited state from which energy may be transferred to the other photophore when in close poximity thereto resulting in excitation and emission of the other photophore. Methods according to the invention involve associating the first photophore-labelled probe with the analyte and associating the second photophore-labelled probe with the analyte or first probe in a reaction mixture bringing the photophores in sufficient proximity to allow energy transfer to occur. The reaction mixture is formed, excited by the modulated energy source and monitored for emission of the photophore excited by energy transfer at a time beyond the emissive lifetime of the shorter-lived photophore.

REFERENCES:
patent: 4058732 (1977-11-01), Wieder
patent: 4261968 (1981-04-01), Ullman
patent: 4542104 (1985-09-01), Stryer

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