Molecular cloning of the genes responsible for collagenase produ

Details Molecular cloning of the genes responsible for collagenase produ Molecular cloning of the genes responsible for collagenase produ

1990-03-22

1993-01-05

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435220, 4353201, 935 14, 935 29, 935 73, 536 237, C12N 1557, C12N 1570, C12N 1531

Patent

active

051770172

ABSTRACT:
Genetically engineered E. coli carry vectors containing inserts that code for Clostridium histolyticum collagenase. These inserts code for: (a) a form of collagenase having a molecular weight of about 68,000 daltons in the essential absence of larger forms of collagenase; (b) the 68 kd form of collagenase and a fusion polypeptide consisting of the collagenase protein fused to at least a portion of the .beta.-galactosidase protein of E. coli; or (3) the 68 kd form of collagenase and polypeptides of molecular weight of from above about 68,000 daltons to about 100,000 daltons and having the enzymatic activity of C. histolyticum collagenase as indicated by digestion of .sup.3 H-acetylated collagen and by specific inhibition by 1,10-phenanthroline plus EDTA. The collagenase genes in the transformed E. coli are expressed efficiently in the transformed cells to yield enzymatically active and immunologically cross-reactive collagenase. In particular, the 68 kd form of collagenase is resistant to autocatalytic degradation and is stable to long-term storage. Genetically engineered collagenase, especia

REFERENCES:
patent: 3705083 (1972-12-01), Chiulli et al.
Biochemistry, vol. 23, No. 22, issued Oct. 23, 1984, Sugasawara, et al., "Purification and Characterization of Three Forms of Collagenase From Clostridium histolyticum", pp. 5175-5181.
Proceedings of the National Academy of Sciences USA, vol. 82, May 1985, Young, et al., "Dissection of Mycobacterium tuberculosis Antigens Using Recombinant DNA", pp. 2583-2587.
Clinical Orthopedics and Related Research, No. 215, Feb. 1987, K. H. Chu, "Collagenase Chemonucleolysis Via Epidural Injection: A Review of 252 Cases", pp. 99-104.
Biochemistry, vol. 27, No. 19, Sep. 20, 1988, Angelton, et al., "Preparation and Reconstitution With Divalent Metal Ions of Class I and Class II Clostridium histolyticum Apocollagenases", pp. 7406-7412.
Biochemistry, vol. 27, No. 19, Sep. 20, 1988, Angleton et al., "Preparation by Direct Metal Exchange and Kinetic Study of Active Site Metal Substituted Class I and Class II Clostridium histolyticum Collagenases", pp. 7413-7418.
M. D. Bond & H. E. Van Wart, "Purification and Separation of Individual Collagenases of Clostridium histolyticum Using Red Dye Ligand Chromatography," Biochemistry 23, 3077-3085 (1984).
M. D. Bond & H. E. Van Wart, "Characterization of the Individual Collagenases from Clostridium histolyticum," Biochemistry 23, 3085-3091 (1984).
M. D. Bond & H. E. Van Wart, "Relationship Between the Individual Collagenases of Clostridium histolyticum: Evidence for Evolution by Gene Duplication," Biochemistry 23, 3092-3099 (1984).
M. F. French, K. A. Mookhtiar & H. E. Van Wart, "Limited Proteolysis of Type I Collagen and Hyperreactive Sites by Class I and II Clostridium histolyticum Collagenases: Complementary Digestion Patterns," Biochemistry 26, 681-687 (1987).
S. K. Mallya, K. A. Mookhtiar & H. E. Van Wart, "Accurate, Quantitative Assays for the Hydrolysis of Soluble Type I, II and III .sup.3 H-Acetylated Collagens by Bacterial and Tissue Collagenases," Anal. Biochem. 158, 334-345 (1986).
K. A. Mooktiar, D. R. Steinbrink and H. E. Van Wart, "Mode of Hydrolysis of Collagen-like Peptides by Class I and Class II Clostridium histolyticum Collagenases: Evidence for Both Endopeptidase and Tripeptidylcarboxypeptidase Activities," Biochemistry 24, 6527-6533 (1985).
K. A. Mookhtiar, S. K. Mallya and H. E. Van Wart, "Properties of Radiolabeled Type I, II, and III Collagens Related to Their Use as Substrates in Collagenase Assays," Anal. Biochem. 158, 322-333 (1986).
H. E. Van Wart & D. R. Steinbrink, "Complementary Substrate Specificities of Class I and Class II Collagenases from Clostridium histolyticum," Biochemistry 24, 6520-6526 (1985).
E. Wunsch & H.-G. Heidrich, "Zur Quantitativen Bestimmung der Kollagenase," Hoppe-Seyler's Zeit. Physiol. Chem. 333, 149-151 (1963).
E. Amann, J. Brosius & M. Ptashne, "Vectors Bearing a Hybrid trp-lac Promoter Useful for Regulated Expression of Cloned Genes in Escherichia coli," Gene 25, 167-178 (1983).
W. N. Burnette, "`Western Blotting`: Electrophoretic Transfer of Proteins from Sodium Dodecyl Sulfate-polyacrylamide Gels to Unmodified Nitrocellulose and Radiographic Detection with Antibody and Radioiodinated Protein A," Anal. Biochem. 112, 195-203 (1981).
D. Hanahan, "Studies on Transformation of Escherichia coli with Plasmids," J. Mol. Biol. 166, 557-580 (1983).
T. V. Huynh, R. A. Young & R. W. Davis, "Constructing and Screening cDNA Libraries in .lambda.gt10 and .lambda.gt11," In DNA Cloning Techniques: A Practical Approach (ed. D. Glover), (IRL Press, Eynsham, 1975, vol. I, pp. 49-79.
M. Russel, S. Kidd & M. R. Kelley, "An Improved Filamentous Helper Phage for Generating Single-stranded Plasmid DNA," Gene 45, 333-338 (1986).
J. Shine & L. Dalgarno, "Determinant of Cistron Specificity in Bacterial Ribosomes," Nature 254, 34-38 (1975).
J. M. Short, J. M. Fernandez, J. A. Sorge & W. D. Huse, ".lambda.ZAP: A Bacteriophage .lambda. Expression Vector with in Vivo Excision Properties," Nucl. Acid. Res. 16, 7583-7600 (1988).
R. A. Young, B. R. Bloom, C. M. Grosskinsky, J. Ivanyi, D. Thomas & R. W. Davis, "Dissection of Mycobacterium tuberculosis Antigens Using Recombinant DNA," Proc. Natl. Acad. Sci. USA 82, 2583, (1985).
R. A. Young & R. W. Davis, "Efficient Isolation of Genes by Using Antibody Probes," Proc. Natl. Acad. Sci. USA 80, 1194-1198 (1983).
R. A. Young & R. W. Davis, "Yeast RNA Polymerase II Genes: Isolation with Antibody Probes," Science 222, 778-782 (1983).
R. A. Young, V. Mehra, D. Sweetser, T. Buchanan, J. Clark-Curtiss, R. W. Davis & B. R. Bloom, "Genes for the Major Protein Antigens of the Leprosy Parasite Mycobacterium leprae," Nature 317, 450-452 (1985).
D. W. R. Gray & P. J. Morris, "Prospects for Pancreatic Islet Transplantation," World J. Surg. 10, 410-421 (1986).
J. S. Najarian, D. E. R. Sutherland, A. J. Matas, M. W. Steffes, R. L. Simmons & F. C. Goetz, "Human Islet Transplantation: A Preliminary Report," Transplantation Proc. 9, 233-236 (1977).
D. W. Scharp, "Isolation and Transplantation of Islet Tissue," World J. Surg. 8, 143-151 (1984).
D. E. R. Sutherland, A. J. Matas, F. C. Goetz & J. S. Najarian, "Transplantation of Dispersed Pancreatic Islet Tissue in Humans: Autografts and Allografts," Diabetes 29, Suppl. 1, 31-44 (1980).
B. J. Sussman, J. W. Bromley & J. C. Gomez, "Injection of Collagenase in the Treatment of Herniated Lumbar Disk: Initial Clinical Report," J. Am. Med. Assn. 245, 730-732 (1981).
R. G. Fisher, J. W. Bromley, G. L. Becker, M. Brown & V. Mooney, "Surgical Experience Following Intervertebral Discolysis with Collagenase," J. Neurosurg. 64, 613-616 (1986).
K. H. Chu, "Collagenase Chemonucleolysis via Epidural Injection: A Review of 252 Cases," Clin. Orthopedics & Related Res. No. 215, 99-104 (Feb. 1987).
Jaye, M., et al., 1983, Nucleic Acids Research, 11(8): 2325-2335.

Affiliated with

Also associated with

Rating

Molecular cloning of the genes responsible for collagenase produ does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Molecular cloning of the genes responsible for collagenase produ, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Molecular cloning of the genes responsible for collagenase produ will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2390388