Multiparametric fluorescence in situ hybridization

Details Multiparametric fluorescence in situ hybridization Multiparametric fluorescence in situ hybridization

1998-06-02

1999-12-28

Brusca, John S.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

060079940

ABSTRACT:
The invention relates to a set of combinatorially labeled oligonucleotide probes each member thereof: (i) having a predetermined label distinguishable from the label of any other member of said set, and (ii) being capable of specifically hybridizing with a predetermined chromosome or nucleic acid molecule, and to the use of such molecules, alone or in concert with nucleic acid amplification methods.

REFERENCES:
Speicher et al. Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nature Genetics vol. 12 pp. 368-375, 1996.
Hackstein et al. The intracellular localization of human cytomegalovirus DNA in peripheral blood leukocytes during active infections by high-resolution fluorescence in situ hybridization. Arch. Virol. vol. 141 pp. 1293-1305, 1996.
Gersdorf et al. Fluorescence in situ hybridization for direct visualization of Gram-negative anaerobes in subgingival plaque samples. FEMS Immunol. Med. Microbiol. vol. 6 pp. 109-114, 1993.

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