Polynucleotide encoding a receptor for a Bacillus thuringiensis

Details Polynucleotide encoding a receptor for a Bacillus thuringiensis Polynucleotide encoding a receptor for a Bacillus thuringiensis

1997-12-10

1999-12-28

Spector, Lorraine

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 6, 435 691, 435325, 4352523, 43525411, 435320, 435 721, 536 231, 536 235, 530350, 530399, G01N 33566, C12N 510, C12N 1511, C07K 14705

Patent

active

060079819

ABSTRACT:
The cDNA that encodes a glycoprotein receptor from the tobacco hornworm which binds a Bacillus thuringiensis toxin has been obtained and sequenced. The availability of this cDNA permits the retrieval of DNAs encoding homologous receptors in other insects and organisms as well as the design of assays for the cytotoxicity and binding affinity of potential pesticides and the development of methods to manipulate natural and/or introduced homologous receptors and, thus, to destroy target cells, tissues and/or organisms.

REFERENCES:
patent: 4675285 (1987-06-01), Clark et al.
patent: 5071654 (1991-12-01), English
Almond et al., Suppression of Protein Structure Destabilizing Mutations in Bacillus thuringiensis .delta.-endotoxin by Second Site Mutations; Biochemistry 32:1040-1046 (1993).
Chen et al., Site-directed mutations in a highly conserved region of Bacillus thuringiensis .delta.-endotoxin affect inhibition of short circuit current across Bombyx mori midguts, Proc. Natl. Acad. Sci. USA 90:9041-9045 (1993).
English, L., Mode of action of delta-endotoxins from Bacillus Thuringiensis: A comparison with other bacterial toxins, Insect Biochem. Molec. Biol. 22(1):1-7.
Gill et al., The Mode of Action of BacillusThuringiensis Endotoxins; Ammu. Rev. Entomol. (1992) 37:615-36.
Hoffman et al., Specificity of Bacillus Thuringiensis .delta.-endotoxins is Correlated with the Presence of High-Affinity Binding Sites in the Brush Border Membrane of Target Insect Midguts; Proc. Natl. Acad. Sci., USA (1988) 85:7844-7848.
Hofte et al., Insecticidal Crystal Proteins of Bacillus Thuringiensis; Microbiological Reviews (1989) 53(2):242-255.
Ishihara et al., Molecular cloning and expression of a cDNA encoding the secretin Receptor, EMOB J., vol. 10, No. 7, 1635-1641.
Knight et al., The receptor for Bacillus thuringiensis CrylA(c) delta-endotoxin in the brush border membrance of the lepidopteran Manduca sexta is aminopeptidase N, Molecular Microbiology (1994) 11(3):429-436.
Knowles et al., The crystal .delta.-endotoxins of Bacillus thuringiensis: Models for their mechanism of action on the insect gut, BioEssays 15(7):469-476 (1993).
Lee et al., Location of a Bombyx mori Receptor Binding Region on a Bacillus thuringiensis .delta.-Endotoxin, J. Biol. Chem., vol. 167, No. 5, pp. 3115-3121 (1992).
Sanchis et al., Identification and partial purification of a Bacillus thuringiensis CryIC .delta.-endotoxin binding protein from Spodoptera littoralis gut membrances, FEBS 316(3):264-268 (1993).
Sangadala et al., A Mixture of Manduca Sectra Aminopeptidase and Phosphatase Enhances Bacillus Thuringiensis Insecticidal CrylA(c) Toxin Binding and Rb.sup.+ -K.sup.+ Efflux in vitro, vol. 269, No. 13, pp. 10088-10092 (1994).
Vadlamudi et al., A Specific Binding Protein from Manduca Sexta for the Insecticidal Toxin of Bacillus Thuringiensis Subsp., Berliner; J. Biol. Chem. 268(17):12334-12340 (1993).
Van Rie et al., Specificity of Bacillus Thuringiensis .delta.-endotoxins; Eur. J. Biochem. (1989) 186:239-247.
Van Rie et al., Receptors on the Brush Border Membrane of the Insect Midgut as Determinants of the Specificity of Bacillus Thuringiensis Belta-Endotoxins (1990) 56(5):1378-1385.
Vadlamundi, R.K., "Cloning and expression of a receptor for an insecticidal toxin of Bacillus thuringiensis", J. Biol. Chem., vol. 270, No. 10, Mar. 10, 1995, pp. 5490-5494.
Dorsch, J. A. et al., "Determination of the specific region of BT-R1 to which the CryaAb toxin of Bacillus thuringiensis subsp. Berliner binds", FASEB Journal (Abstracts), vol. 11, No. 9, Jul. 31, 1997, p. A1050.

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