Preferential signal production on a surface in immunoassays

Details Preferential signal production on a surface in immunoassays Preferential signal production on a surface in immunoassays

1979-12-26

1981-11-10

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 7, 435805, 435810, 422 56, 23230B, 424 12, G01N 3354, C12Q 168, G01N 148

Patent

active

042999162

ABSTRACT:
An assay method and compositions are provided for determining the presence of an analyte in a sample. The analyte is a member of an immunological pair (mip) of immunogens--ligand and receptor. The method has two basic elements: a solid surface to which one of the members of the immunological pair is bonded and a signal producing system, which includes a catalytic member bonded to a mip, which signal producing system results in a measurable signal on said solid surface related to the amount of analyte in the medium. The signal generating compound is produced without separation of the catalyst labeled mip bound to the solid surface from the catalyst labeled mip free in solution.
In a preferred embodiment, an enzyme is bonded to a mip which acts in conjunction with a solute to produce a signal generating product which binds preferentially to the solid surface when the enzyme is bound to the surface, resulting in a signal which is readily differentiated from signal generating compound produced by the catalyst and solute in the bulk solution.

REFERENCES:
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 4059407 (1977-11-01), Hochstrasser et al.
patent: 4067959 (1978-01-01), Bolz
patent: 4071315 (1978-01-01), Chateau
patent: 4094647 (1978-06-01), Deutsch et al.
patent: 4168146 (1979-09-01), Grubb et al.
patent: 4184920 (1980-01-01), Blixt et al.
patent: 4208479 (1980-06-01), Zuk et al.

Affiliated with

Also associated with

Rating

Preferential signal production on a surface in immunoassays does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Preferential signal production on a surface in immunoassays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Preferential signal production on a surface in immunoassays will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2363149