7-Deaza-2'-deoxyguanosine nucleotides and nucleic acids analogs

Details 7-Deaza-2'-deoxyguanosine nucleotides and nucleic acids analogs 7-Deaza-2'-deoxyguanosine nucleotides and nucleic acids analogs

1988-11-14

1996-01-02

Robinson, Douglas W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2623, 536 2626, 536 267, 536 2714, 536 272, 536 2781, 536 282, 435 6, C07H 19173, C07H 2104, C07H 1920

Patent

active

054809800

ABSTRACT:
The present invention provides 7-deaza-2'-deoxy-guanosine nucleotides of the general formula: ##STR1## wherein R is a --PO.sub.3 H.sub.2, --P.sub.2 O.sub.6 H.sub.3 or --P.sub.3 O.sub.9 H.sub.4 residue or an alkali metal, alkaline earth metal or ammonium salt of the phosphate groups.
The present invention also provides processes for the preparation of these nucleotides and is also concerned with the use thereof in the sequencing of DNA.

REFERENCES:
patent: 3337530 (1967-08-01), Hanze
patent: 4500707 (1985-02-01), Caruthers et al.
patent: 4804748 (1989-02-01), Seela
Winkeler et al., "Synthesis of 2-Amino-7-(2'-deoxy-.beta.-D-erythro-pentofuranosyl)-3,7-dihydro-4H-pyrrol o[2,3-d]pyrimidin-4-one, a New Isostere of 2'-Deoxyguanosine," J. Org. Chem., 48(18), 3119-3122 (1983).
Barr et al., "7-Deaza-2'-Deoxyfuanosine-5'-Triphosphate: Enhanced Resolution in M13 Dideoxy Sequencing," BioTechniques, 4(5), 428-432 (1986).
Mizusawa et al., "Improvement of the Dideoxy Chain Termination Method of DNA Sequencing by Use of Deoxy-7-deazaguanosine Triphosphate in Place of GTP," Nucleic Acids Research, 14(3), 1319-1324 (1986).
Sanger et al., "DNA Sequencing with Chain-Terminating Inhibitors," Proc. Nat. Acad. Sci. USA, 74(12), 5463-5467 (1977).
Seela et al., "Poly(7-deazaguanylic acid), the Homopolynucleotide of the Parent Nucleoside of Queosine," Biochemistryr, 21(18), 4338-4343 (1982).
Sen et al., "A Sodium-Potassium Switch in the Formation of Four-Stranded G4-DNA," Nature, 344, 410-414 (1990).

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