Method of producing human monoclonal antibodies and kit therefor

Details Method of producing human monoclonal antibodies and kit therefor Method of producing human monoclonal antibodies and kit therefor

1994-10-17

1996-10-22

Ziska, Suzanne E.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4351722, C12N 500, C12N 1500

Patent

active

055676103

ABSTRACT:
A method for the removal of, for in vitro immunization, undesired cells from human lymphocyte containing cell populations is disclosed. For the method, use is made of lysosomotropic agents which are caused to act in vitro on human lymphocyte containing cell populations.
Also disclosed is a method in the in vitro immunization of lymphocytes for use in the production of human monoclonal antibodies. In this method, lysosomotropic agents are caused to act in vitro on human lymphocyte containing cell populations for removing cell populations having a negative effect on the in vitro immunization, whereupon the lymphocytes are in vitro immunized antigen-specifically and immortalized.
Finally, a kit for in vitro immunization of lymphocytes for use in the production of human monoclonal antibodies is disclosed. The kit comprises as active constituents a container containing lymphokines and a container containing lysosomotropic agents.

REFERENCES:
patent: 4752602 (1988-06-01), Lipsky
Thiele et al J. Immunology 136(3): 1038, 1986.
Hoffman et al in Principle of In Vitro Immunization of Human Blymplocyts, pp. 277-289. Plenun Press 1985.
Borrebaeck, C. A. K. (1986) Tibtech, 49 147.
Borrebaeck, C. A. K. & Moeller, S. A. (1986) J. Immunol. 136, 3710.
Danielsson, L., Moeller S. A. & Borrebaeck, C. A. K. (1986) Immunology 61, 51.
Goldman, R. & Kaplan, A. (1973) Biochim.Biophys. Acta 318, 205.
Ho, M. K., Rand, N. Murray, J., Kato, K. & Rabin, H. (1985) J.Immunol.135,3831.
Hoffman, M. K. & Hirst, J. A. (1985) Principles of in Vitro Immunization of Human B Lymphocytes, pp. 277-289, Plenum Press.
Strike, L. E., Devens, B. H. & Lundak, R. L. (1984)J.Immunol.132, 1798.
Teng, N. N. H., Reyes, G. R., Bieber, M., Fry, K. E., Lam, K. S. & Herbert, J. M.(1985) in Human Hybrodomas and Monoclonal Antibodies (Engleman et al Eds), 71-91, Plenum Press.
Thiele, D. L. & Lipsky, P. E.(1986) J. Immunol. 136, 1038.
Thiele, D. L., Kurosaka, M. & Lipsky, P. E.(1983) J.Immunol. 131, 2282 Chemical Abstracts, vol. 100:84116m, 1984, p. 422.
Medline, NLM Accession No 84034197, J.Immunol. 1983 Nov; 131(5):2282-90.
Chemical Abstracts, vol. 105: 72232m, 1986, Cancer Res.1986, 46(7) 3295-8.
Chemical Abstracts, vol. 96: 117049w, 1982, Dev.Biol.1982, 90(1), 91-8.
Thiele et al, Proc. Natl. Acad. Sci. USA, vol. 82, pp. 2468-2472 (1985).
Thiele et al, The Journal of Immunology, vol. 134, No. 2, pp. 786-793 (1985).

Affiliated with

Also associated with

Rating

Method of producing human monoclonal antibodies and kit therefor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of producing human monoclonal antibodies and kit therefor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of producing human monoclonal antibodies and kit therefor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2358064