Nucleic acid sequence amplification methods

Details Nucleic acid sequence amplification methods Nucleic acid sequence amplification methods

1990-07-10

1996-01-02

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, C12P 1934

Patent

active

054807840

ABSTRACT:
Methods of synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. Nucleotide sequences of target nucleic acid portions and of primers are selected to minimize the ability of the primer to remain able to form a DNA primer extension product of for formation of an RNA:DNA primer hybrid and exposure to RNase H.

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