Method for reducing background signals in DNA replication/detect

Details Method for reducing background signals in DNA replication/detect Method for reducing background signals in DNA replication/detect

1994-03-31

1996-01-02

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435183, 435 911, 536 231, 536 2433, 536 253, 935 16, 935 76, 935 77, 935 78, C12P 1934, C12Q 168, C07H 2104, C12N 1500

Patent

active

054807832

ABSTRACT:
A kit and method for reduction of primer-independent background signals in a DNA replication/detection assay, particularly in a direct in situ PCR amplification/detection assay, for a target DNA sequence in DNA sample, comprising pretreating the DNA sample, containing the target DNA sequence to be replicated/amplified and assayed, with DNA polymerase and one or more dideoxynucleoside triphosphates prior to conducting the replication/amplification steps.

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