Rhodamine phosphoramidite compounds

Details Rhodamine phosphoramidite compounds Rhodamine phosphoramidite compounds

1990-10-22

1993-07-27

Rollins, John W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

549223, 549227, 536 243, C07F 900

Patent

active

052311910

ABSTRACT:
The invention provides a novel cleavage reagent for hydrolysing base-labile linking groups between a solid phase support and oligonucleotides. The cleavage reagent comprises a lower alkyl alcohol, water, and a non-nuccleophilic hindered alkylamine containing from 3 to 6 carbon atoms in a ratio of about 1:1:1 to about 1:3:1, respectively. An important property of the cleavage reagent is that it preserves the fluorescent characteristics of rhodamine dyes during cleavage, thereby making it possible to completely synthesize rhodamine-labeled oligonucleotides by solid phase methods. Rhodamine phosphoramidites are provided to further enhance the efficiency of synthesizing rhodamine-labeled oligonucleotides by such methods.

REFERENCES:
patent: 2153059 (1939-04-01), Eckert et al.
patent: 2242572 (1941-05-01), Eckert et al.
patent: 3822270 (1974-07-01), Reynolds
patent: 4005092 (1977-01-01), Reynolds
patent: 4965349 (1990-10-01), Woo et al.

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