Method of inserting amino acid analogs into proteins

Details Method of inserting amino acid analogs into proteins Method of inserting amino acid analogs into proteins

1983-03-21

1986-02-04

Marantz, Sidney

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

260112R, 2601125R, 435 68, 935 3, 935 20, 935111, C12P 2102

Patent

active

045686408

ABSTRACT:
A method for substituting one amino acid for another in a protein chain to improve selected properties of the protein. Initially, an mRNA molecule capable of producing an unmodified protein of the type it is desired to modify is provided. Next the codon along the mRNA chain which ordinarily accepts the anti-codon corresponding to a first amino acid which is to be replaced with a selected second amino acid is determined. The tRNA which ordinarily brings the first amino acid to that site is modified to carry the second amino acid instead. The modified protein is then formed by translation techniques in the presence of the modified tRNA, whereby the second amino acid is incorporated in the protein chain in place of the first amino acid. Several methods of accomplishing the modification of the selected tRNA are also disclosed.

REFERENCES:
patent: 4351901 (1982-09-01), Bahl
patent: 4469631 (1984-09-01), Baxter
Jelenc, P. C. et al., Proc. Natl. Acad. Sci. USA, 76(7), 3174-3178, (Jul. 1979).

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