Separation of high-activity heparin by affinity chromatography

Details Separation of high-activity heparin by affinity chromatography Separation of high-activity heparin by affinity chromatography

1977-08-22

1978-10-24

Marantz, Sidney

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C08B 3710

Patent

active

041222504

ABSTRACT:
An affinity chromatography method for the separation of high-activity heparin from a mixture thereof with low-activity heparin. The method employs an affinity column of DEAE-Sephadex and a series of sodium chloride-imidazole elution buffers (pH 6.5-7.5) varying in sodium chloride molarity from about 0.5 to about 1.0. Stepwise elution of a plurality of heparin fractions from the affinity column with the elution buffers of successively increasing sodium chloride molarity results in the removal from the affinity column of first the low-activity heparin and finally the high-activity heparin.

REFERENCES:
patent: 3099600 (1963-07-01), Toccaceli
R. Bohn et al., J. Chromatogr., 62(3), 399-408 (1971).
R. Bohn et al., J. Chromatogr., 62(3), 409-415 (1971).

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