Methods for screening for inhibitors of the transcription-enhanc

Details Methods for screening for inhibitors of the transcription-enhanc Methods for screening for inhibitors of the transcription-enhanc

1994-12-07

2000-04-18

Wortman, Donna C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 78, 435 6, 436501, C12Q 170, G01N 3353, G01N 33566

Patent

active

06051373&

ABSTRACT:
Disclosed is a method for screening for inhibitors of Hepatitis B Virus pX activity. The method involves contacting a test compound with (I) the pX protein of HBV, (ii) a transcription factor comprising the bZIP domain, or fragments that comprise a minimal a bZIP domain, and (iii) an oligoduplex comprising a target DNA sequence of the transcription factor to form a test mixture. After incubating the test mixture under appropriate conditions and for a sufficient time to allow pX-mediated dimerization and DNA binding of the transcription factor to occur, the level of DNA binding of the transcription factor in each test mixture is determined. A test compound is considered to be any compound that causes a decrease in the level of DNA binding in the test mixture relative to the level of DNA binding in control mixtures.

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