Method of making oligonucleotides, oligonucleoside thiophosphate

Details Method of making oligonucleotides, oligonucleoside thiophosphate Method of making oligonucleotides, oligonucleoside thiophosphate

1993-08-13

1996-08-20

Robinson, Douglas W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 253, 536 231, 536 245, C07H 2100

Patent

active

055480764

ABSTRACT:
A method is provided for the high fidelity, rapid and economical in vitro synthesis of oligonucleotides. Nucleoside H-phosphonates are condensed in seriatim using a dehydrating agent to produce a poly (nucleoside H-phosphonate). The product is oxidized to yield the desired oligonucleotide. A novel reagent is provided for multiple nucleoside additions in single cycles.

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