Fermentation processes for preparing soluble ricin A

Details Fermentation processes for preparing soluble ricin A Fermentation processes for preparing soluble ricin A

1994-03-28

1996-08-20

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435691, 530412, C12N 922, C12N 1529, C12P 2102, C07K 14415

Patent

active

055478670

ABSTRACT:
Processes for preparing polypeptides such as ricin A such that soluble polypeptide may be obtained. The pH may be adjusted during the process or the temperature decreased during the terminal portion of the cultivation, and yeast extract may be added to the growth medium during the cultivation. In the case of ricin A the pH is generally adjusted by lowering the pH from a first value such as 6.7 to a second value such as a value between about 5.5 and 6.7.

REFERENCES:
patent: 4689401 (1987-08-01), Ferris
patent: 4894334 (1990-01-01), Ben-Bassat et al.
L. B. Tsai `The Effect Of Organic Nitrogen And Glucose On The Production Of Recombinant Human Insulin-Like Growth Factor In High Cell Density Escherichia Coli Fermentations` Journal Of Indus Microbiology, Aug. 1987, pp. 181-186.
LI, `The Production Of Recombinant Beta-Galactosidase In Escherichia Coli In Yeast Extract Enriched Medium` Journal For Industrial Microbiology, 1990 pp. 85-93.
O'Hare, et al (1987) Febs Letters 216 73-78.
Wilkinson et al (1991) Biotechnology 9 443-448.
Schein (1989) Biotechnology 7 1141-1149.
Piatak et al (1988) J. Biological Chemistry 263 4837-4843.
Kopetzki et al (1989) Mol. Gen. Gevet. 216 149-155.

Affiliated with

Also associated with

Rating

Fermentation processes for preparing soluble ricin A does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fermentation processes for preparing soluble ricin A, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fermentation processes for preparing soluble ricin A will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2330192