Method for the isolation and purification of vitamin K-dependent

Details Method for the isolation and purification of vitamin K-dependent Method for the isolation and purification of vitamin K-dependent

1995-02-27

1997-05-27

Cain, Edward J.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

530380, 530382, 530384, 530413, 530416, A61K 3514

Patent

active

056333501

ABSTRACT:
A method for the separation of vitamin K-dependent proteins from non-vitamin K-dependent accompanying proteins is described wherein the method is characterized in that at least anion exchange chromatography and optionally affinity chromatography is carried out as well. The method is suitable especially for the purification of Factor II, VII, IX, X as well as Protein S, Protein C and Protein Z. With the aid of the method according to the invention a vitamin K-dependent protein is obtained which is present at a purity of 95%.

REFERENCES:
patent: 5330907 (1994-07-01), Philapitsch et al.

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