Method for mass spectrometric analysis of samples from electroph

Details Method for mass spectrometric analysis of samples from electroph Method for mass spectrometric analysis of samples from electroph

1995-03-09

1997-01-21

Phasge, Arun S.

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204462, 204613, 204614, G01N 2726, G01N 27447

Patent

active

055956360

ABSTRACT:
The invention relates to the mass spectrometric analysis of separated substance samples on so-called 2-D-gel electrophoresis plates, used particularly for protein determination, with ionization of the substance samples by MALDI. The molecules of the substance samples are transferred to a thin, lacquer-like smooth matrix layer which should preferably be applied to a metal sample support. Transfer takes place directly or indirectly, for example via a polyvinylidene difluoride membrane an an intermediate carrier, by electrophoretic transport of the molecules to the matrix surface. Before transfer, the proteins may be subjected to enzymatic cutting of their amino acid chains.

REFERENCES:
patent: 5260571 (1993-11-01), Cottrell et al.
Kerstin Strupat et al., Matrix-Assisted Laser Desorption Ionization Mass Spectrometry of Proteins Electroblotted after Polyacrylamide Gel Electrophoresis, Analytical Chemistry, vol. 66, pp. 464-470, No. 4, Feb. 14, 1994.
Martha M. Vestling and Catherine Fenselau, Poly (vinylidene diflouride) Membranes as the Interface between Laser Desorption Mass Spectrometry, Gel Electrophoresis and in Situ Proteolysis, Analytical Chemistry, vol. 66, No. 4, pp. 471-477, Feb. 15, 1994.

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